1. Laser Capture Microdissection: Insights into Methods and Applications
Meera Mahalingam
2. Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues
Rémi Longuespée, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, and Edwin De Pauw
3. Isolation of DNA and RNA from Laser Microdissected Tissue Samples
Yelena G. Golubeva and Andrew C. Warner
4. Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE
DaRue A. Prieto, Gordon Whitely, Donald J. Johann, Jr., and Josip Blonder
5. Next Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens
Lavinia Mägel, Stephan Bartels, and Ulrich Lehmann
6. Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model
Nadia P. Castro and Yelena G. Golubeva7. Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease
Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio, and Giuseppe Mazzarella
8. Laser Capture Microdissection and Isolation of High Quality RNA from Frozen Endometrial Tissue
Michele Cummings, Georgia Mappa, and Nicolas M. Orsi
9. Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions
Monica Molano, Suzanne M. Garland, and Alyssa M. Cornall
10. Laser Capture Microdissection and Transcriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone
Efrain Pacheco, Rong Hu, and Scott Taylor
11. Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain
Sarah A. Mauney, Tsung-Ung W. Woo, and Kai C. Sonntag
12. The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience
Renée Morris and Prachi Mehta
13. Laser Capture Microdissection in Traumatic Brain Injury Research: Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses
Deborah R. Boone, Harris A. Weisz, Stacy L. Sell, and Helen L. Hellmich14. Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High Performance Liquid Chromatography - Mass Spectrometry
Luisa Aring, Simone Steinbach, Katrin Marcus, and Caroline May
15. Immuno-Guided Laser Capture Microdissection of Glial Cells for mRNA Analysis
Arnaud B. Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, and David A. Laplaud
16. Immuno-Laser Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post Mortem Human Brain
Julie E. Simpson, Stephen B. Wharton, and Paul R. Heath
17. Laser Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR
Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, and Eduardo Molina-Holgado
18. Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer's Disease Tissue Using Laser Capture Microdissection for Downstream Proteomics
Eleanor Drummond, Shruti Nayak, Geoffrey Pires, Beatrix Ueberheide, and Thomas Wisniewski19. Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post Mortem Midbrain Samples by UV-
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques. 428 pp. Englisch. N° de réf. du vendeur 9781493975570
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Buch. Etat : Neu. Neuware -This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 428 pp. Englisch. N° de réf. du vendeur 9781493975570
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques. N° de réf. du vendeur 9781493975570
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