Synopsis
1. Neurotrophic Factors: An Overview
Stephen D. Skaper
2. Ocular Nerve Growth Factor (NGF) and NGF Eye Drop Application as Paradigms to Investigate NGF Neuroprotective and Reparative Actions
Paola Tirassa, Pamela Rosso, and Angela Iannitelli
3. Culture of Rodent Cortical, Hippocampal, and Striatal Neurons
Laura Facci and Stephen D. Skaper
4. Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex, Spinal Cord, and Cerebellum
Stephen D. Skaper and Laura Facci
5. Central Nervous System Neuron-Glia Co-Culture Models and Application to Neuroprotective Agents
Stephen D. Skaper and Laura Facci
6. Preparation of Adult Rat Sensory Neuron Cultures and Their Application to Growth Cone Turning Assays
Christine A. Webber and Douglas W. Zochodne
7. Sensory Neurons from Tau Transgenic Mice and Their Utility in Drug Screening
Aviva M. Tolkovsky and Jack Brelstaff
8. Culture of Rat Mesencephalic Dopaminergic Neurons and Application to Neurotoxic and Neuroprotective Agents
Stephen D. Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, and Laura Facci
9. Culture of Neurons and Smooth Muscle Cells from the Myenteric Plexus of Adult Mice
Paola Brun and Hamid I. Akbarali
10. Astrocyte/Microglia Co-Cultures as a Model to Study Neuroinflammation
Laura Facci, Massimo Barbierato, and Stephen D. Skaper
11. Micropatterned Geometries Shape Oligodendrocyte and Microglia Plasticity
Cinzia Volonté, Adele De Ninno, and Susanna Amadio
12. Oligodendrocyte Progenitor Cell Cultures: A Model to Screen Neurotrophic Compounds for Myelin Repair
Massimo Barbierato, Stephen D. Skaper, and Laura Facci
13. Cell Enumeration Assays: Application of the MTT and Sulforhodamine B Assays to Lipopolysaccharide-Stimulated Neonatal Rodent Microglia
Laura Facci and Stephen D. Skaper
14. Diolistic Labeling and Analysis of Dendritic Spines
M. Foster Olive, Armani P. Del Franco, and Cassandra D. Gipson
15. Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses
Dimitar Dimitrov, Laurent Guillaud, Kohgaku Eguchi, and Tomoyuki Takahashi
16. Methods for Quantitative Analysis of Axonal Cargo Transport
Matias Alloatti, Luciana Bruno, and Tomas L. Falzone
17. Neuronal Cell Morphology in Primary Cerebellar Granule Cells Using High-Content Analysis
Nicholas Radio and Steven Frank
18. Nerve Growth Factor-Induced Angiogenesis: 1. Endothelial Cell Tube Formation Assay
Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Hacham, Arnon Fluksman, Ofra Benny, Cezary Marcinkiewicz, and Peter I. Lelkes
19. Nerve Growth Factor-Induced Angiogenesis: 2. The Quail Chorioallantoic Membrane Assay
Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Cezary Marcinkiewicz, and Peter I. Lelkes
20. Rat Pancreatic Beta-Cell Culture
Myrian Velasco, Carlos Larqué, Carlos Manlio Díaz-García, Carmen Sanchez-Soto, and Marcia Hiriart
21. Growth and Neurotrophic Factors in Embryonic Stem Cells
Micaela Pannella, Luciana Giardino, Laura Calzà, and Mercedes
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Neurotrophic Factors. Methods and Protocols.
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New York, Humana Press., 2018
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Gebunden. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocols for the study of neurotrophic factorsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts. N° de réf. du vendeur 168579081
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Neurotrophic Factors
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology. 476 pp. Englisch. N° de réf. du vendeur 9781493975709
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Neurotrophic Factors | Methods and Protocols
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Buch. Etat : Neu. Neurotrophic Factors | Methods and Protocols | Stephen D. Skaper | Buch | xv | Englisch | 2017 | Springer New York | EAN 9781493975709 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. N° de réf. du vendeur 110974912
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Neurotrophic Factors
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ISBN 10 : 1493975706
ISBN 13 : 9781493975709
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Buch. Etat : Neu. Neuware -This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 476 pp. Englisch. N° de réf. du vendeur 9781493975709
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Neurotrophic Factors : Methods and Protocols
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Springer New York, Springer New York, 2017
ISBN 10 : 1493975706
ISBN 13 : 9781493975709
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology. N° de réf. du vendeur 9781493975709
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Neurotrophic Factors: Methods and Protocols (Methods in Molecular Biology)
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