Description du livre PAP. Etat : New. New Book. Shipped from UK. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. N� de r�f. du vendeur L0-9783659393853

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Monoclonal antibodies anti-beta2-glycoprotein I

Carolina Nigro Stella

Edit� par LAP LAMBERT Academic Publishing Mai 2013 (2013)

ISBN 10 : 3659393851 ISBN 13 : 9783659393853

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Description du livre Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Beta2-glycoprotein I ( 2GPI) detection is important for the accurate diagnosis of autoimmune and chronic inflammatory disease. This book describes the main steps towards a successful obtaining, initial characterization, analytical applications and lab-scale production of a set of anti- 2GPI monoclonal antibodies. The 2GPI antigen purification was comprehensively explored and six different hybridomas were obtained after cell fusion of immunized mouse lymphocytes with SP2-O murine myeloma cells. The antibodies were purified after hybridomas expansion from ascites (in vivo) and conventional cell culture (in vitro) and their reactivity was evaluated towards the 2GPI antigen either adsorbed to solid phase support (Dot-Blot, immunoblotting, direct ELISA), or in solution (indirect ELISA). 2GPI recognition was achieved with one of the clones in paraffin embedded tissues (immunohistochemistry), and in blood and lymph (ELISA). The application of 2GPI recognition to a sepsis and bacterial translocation animal model granted an unexpected and exciting sequence of achievements concerning the pathophysiology of autoimmunity control by this protein. 92 pp. Englisch. N� de r�f. du vendeur 9783659393853

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Monoclonal antibodies anti-beta2-glycoprotein I : Production, characterization, analytical applications

Carolina Nigro Stella

Edit� par LAP LAMBERT Academic Publishing (2013)

ISBN 10 : 3659393851 ISBN 13 : 9783659393853

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AHA-BUCH GmbH
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Description du livre Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Beta2-glycoprotein I ( 2GPI) detection is important for the accurate diagnosis of autoimmune and chronic inflammatory disease. This book describes the main steps towards a successful obtaining, initial characterization, analytical applications and lab-scale production of a set of anti- 2GPI monoclonal antibodies. The 2GPI antigen purification was comprehensively explored and six different hybridomas were obtained after cell fusion of immunized mouse lymphocytes with SP2-O murine myeloma cells. The antibodies were purified after hybridomas expansion from ascites (in vivo) and conventional cell culture (in vitro) and their reactivity was evaluated towards the 2GPI antigen either adsorbed to solid phase support (Dot-Blot, immunoblotting, direct ELISA), or in solution (indirect ELISA). 2GPI recognition was achieved with one of the clones in paraffin embedded tissues (immunohistochemistry), and in blood and lymph (ELISA). The application of 2GPI recognition to a sepsis and bacterial translocation animal model granted an unexpected and exciting sequence of achievements concerning the pathophysiology of autoimmunity control by this protein. N� de r�f. du vendeur 9783659393853

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Monoclonal antibodies antibeta2glycoprotein I Production, characterization, analytical applications

Stella Carolina Nigro

Edit� par LAP LAMBERT Academic Publishing (2013)

ISBN 10 : 3659393851 ISBN 13 : 9783659393853

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Description du livre PAP. Etat : New. New Book. Delivered from our UK warehouse in 4 to 14 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. N� de r�f. du vendeur L0-9783659393853

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