Biochemical characterization of polyphenol oxidase (PPO): A reference Book: Includes protocols for extraction and partial characterization of PPO from Artocarpus altilis fruit

 
9783659757181: Biochemical characterization of polyphenol oxidase (PPO): A reference Book: Includes protocols for extraction and partial characterization of PPO from Artocarpus altilis fruit
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Polyphenol oxidases are a group of enzymes widely found in bacteria,fungi,plants and animals.The study was conducted to isolate and characterise Polyphenol oxidase from Artocarpus altilis fruit (bread fruit).In plant chloroplasts, these enzymes are usually found, although they get released from this compartment during senescence or ripening.This enzyme is responsible for the browning of many fruits and vegetables,creating an economic loss to the farmers. Many researchers have worked on extraction and characterization of this enzyme from different plants and still working on its inhibition to prevent the loss.In this project, we have made an attempt to extract the enzyme polyphenol oxidase from the pulp of bread fruit and characterise it.The study has revealed the presence of active Polyphenol oxidase in the pulp of A. altilis which has high specificity for catechol compared to phenol, whose activity lost by TCA, which has optimum pH of 6,optimum temperature of 35 degree celcius,the SDS-PAGE and Zymography technique was employed to determine the molecular weight of enzyme. The forgoing account gives brief insights for future research.

Biographie de l'auteur :

Makari Hanumanthappa is serving as Assistant Professor and Head in the Department of Biotechnology, IDSG Government College, Chikmagalur, India. He has published more than 30 research and review papers in reputed journals and has been serving as reviewer for many scientific journals. He is pursuing PhD in Microbiology from Bharathiar University.

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Chandana Mavinakere; Hanumanthappa K. Makari; K. V. Aanjana Devi
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Chandana Mavinakere; Hanumanthappa K. Makari; K. V. Aanjana Devi
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Description du livre LAP Lambert Academic Publishing, United States, 2015. Paperback. État : New. 229 x 152 mm. Language: English . Brand New Book ***** Print on Demand *****.Polyphenol oxidases are a group of enzymes widely found in bacteria, fungi, plants and animals.The study was conducted to isolate and characterise Polyphenol oxidase from Artocarpus altilis fruit (bread fruit).In plant chloroplasts, these enzymes are usually found, although they get released from this compartment during senescence or ripening.This enzyme is responsible for the browning of many fruits and vegetables, creating an economic loss to the farmers. Many researchers have worked on extraction and characterization of this enzyme from different plants and still working on its inhibition to prevent the loss.In this project, we have made an attempt to extract the enzyme polyphenol oxidase from the pulp of bread fruit and characterise it.The study has revealed the presence of active Polyphenol oxidase in the pulp of A. altilis which has high specificity for catechol compared to phenol, whose activity lost by TCA, which has optimum pH of 6, optimum temperature of 35 degree celcius, the SDS-PAGE and Zymography technique was employed to determine the molecular weight of enzyme. The forgoing account gives brief insights for future rese. N° de réf. du libraire AAV9783659757181

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Chandana Mavinakere; Hanumanthappa K. Makari; K. V. Aanjana Devi
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Chandana Mavinakere; Hanumanthappa K. Makari; K. V. Aanjana Devi
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Chandana Mavinakere; Hanumanthappa K. Makari; K. V. Aanjana Devi
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Description du livre LAP Lambert Academic Publishing Okt 2015, 2015. Taschenbuch. État : Neu. 220x150x3 mm. Neuware - Polyphenol oxidases are a group of enzymes widely found in bacteria,fungi,plants and animals.The study was conducted to isolate and characterise Polyphenol oxidase from Artocarpus altilis fruit (bread fruit).In plant chloroplasts, these enzymes are usually found, although they get released from this compartment during senescence or ripening.This enzyme is responsible for the browning of many fruits and vegetables,creating an economic loss to the farmers. Many researchers have worked on extraction and characterization of this enzyme from different plants and still working on its inhibition to prevent the loss.In this project, we have made an attempt to extract the enzyme polyphenol oxidase from the pulp of bread fruit and characterise it.The study has revealed the presence of active Polyphenol oxidase in the pulp of A. altilis which has high specificity for catechol compared to phenol, whose activity lost by TCA, which has optimum pH of 6,optimum temperature of 35 degree celcius,the SDS-PAGE and Zymography technique was employed to determine the molecular weight of enzyme. The forgoing account gives brief insights for future research. 56 pp. Englisch. N° de réf. du libraire 9783659757181

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Chandana Mavinakere; Hanumanthappa K. Makari; K. V. Aanjana Devi
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ISBN 10 : 3659757187 ISBN 13 : 9783659757181
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Description du livre LAP Lambert Academic Publishing Okt 2015, 2015. Taschenbuch. État : Neu. 220x150x3 mm. Neuware - Polyphenol oxidases are a group of enzymes widely found in bacteria,fungi,plants and animals.The study was conducted to isolate and characterise Polyphenol oxidase from Artocarpus altilis fruit (bread fruit).In plant chloroplasts, these enzymes are usually found, although they get released from this compartment during senescence or ripening.This enzyme is responsible for the browning of many fruits and vegetables,creating an economic loss to the farmers. Many researchers have worked on extraction and characterization of this enzyme from different plants and still working on its inhibition to prevent the loss.In this project, we have made an attempt to extract the enzyme polyphenol oxidase from the pulp of bread fruit and characterise it.The study has revealed the presence of active Polyphenol oxidase in the pulp of A. altilis which has high specificity for catechol compared to phenol, whose activity lost by TCA, which has optimum pH of 6,optimum temperature of 35 degree celcius,the SDS-PAGE and Zymography technique was employed to determine the molecular weight of enzyme. The forgoing account gives brief insights for future research. 56 pp. Englisch. N° de réf. du libraire 9783659757181

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Chandana Mavinakere; Hanumanthappa K. Makari; K. V. Aanjana Devi
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Chandana Mavinakere; Hanumanthappa K. Makari; K. V. Aanjana Devi
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ISBN 10 : 3659757187 ISBN 13 : 9783659757181
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Description du livre LAP Lambert Academic Publishing Okt 2015, 2015. Taschenbuch. État : Neu. 220x150x3 mm. This item is printed on demand - Print on Demand Neuware - Polyphenol oxidases are a group of enzymes widely found in bacteria,fungi,plants and animals.The study was conducted to isolate and characterise Polyphenol oxidase from Artocarpus altilis fruit (bread fruit).In plant chloroplasts, these enzymes are usually found, although they get released from this compartment during senescence or ripening.This enzyme is responsible for the browning of many fruits and vegetables,creating an economic loss to the farmers. Many researchers have worked on extraction and characterization of this enzyme from different plants and still working on its inhibition to prevent the loss.In this project, we have made an attempt to extract the enzyme polyphenol oxidase from the pulp of bread fruit and characterise it.The study has revealed the presence of active Polyphenol oxidase in the pulp of A. altilis which has high specificity for catechol compared to phenol, whose activity lost by TCA, which has optimum pH of 6,optimum temperature of 35 degree celcius,the SDS-PAGE and Zymography technique was employed to determine the molecular weight of enzyme. The forgoing account gives brief insights for future research. 56 pp. Englisch. N° de réf. du libraire 9783659757181

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