Translesion DNA Polymerases: Targeting Translesion DNA Synthesis in the Context of Cancer Therapy - Couverture souple

Knobel, Philip Alexander

Couverture souple

ISBN 10 : 3838120523 ISBN 13 : 9783838120522

Editeur : Südwestdeutscher Verlag für Hochschulschriften, 2011

Synopsis

While investigating the involvement of REV3, the catalytic subunit of TLS Polymerase zeta (Pol ζ) in the development of chemotherapy resistance, it was found that inhibition of REV3 expression per se suppresses colony formation of cancer cells whereas normal cells were less affected. It was confirmed that inhibition of REV3 in various cancer cell lines leads to a growth inhibition, due to accumulation of persistent DNA damage, subsequently leading to the activation of the ATM-dependent DNA damage response (DDR) cascade. Inhibition of REV3 in p53-proficient cancer cells results in a G1-arrest and senescence. In contrast, the inhibition of REV3 in p53-deficient or p53-knockout cancer cells results in a growth inhibition and a G2/M-arrest whereas a small fraction of the p53-deficient or p53-knockout cancer cells can overcome the G2/M-arrest, which results in mitotic slippage and aneuploidy. It is shown for the first time that inhibition of a TLS Pol confers synthetic sickness/lethality specifically in cancer cells. The inhibition REV3 per se suppresses cancer cell growth whereas normal cells are less affected, thus identifying REV3 as a potential target for a cancer-specific therapy.

A propos du livre

À propos de l'auteur

Dissertation and study of Biology at the University of Zurich, Switzerland. Postdoctoral fellow at the Institute for Research in Biomedicine in Barcelona, Spain.

Détails de l'édition

• Éditeur: Südwestdeutscher Verlag für Hochschulschriften
• Date d'édition: 2011
• Langue: anglais
• ISBN 10: 3838120523
• ISBN 13: 9783838120522
• Reliure: Broché
• Nombre de pages: 92
• Coordonnées du fabricant: non disponible
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