Calcium Techniques: A Laboratory Manual

Parys, Jan B. (Editor)/ Bootman, Martin (Editor)/ Yule, David I. (Editor)/ Bultynck, Geert (Editor)

ISBN 10: 1936113589 / ISBN 13: 9781936113583

Edité par Cold Spring Harbor Laboratory Pr, 2013

lab manual edition. 592 pages. 12.00x10.00x1.00 inches. In Stock. N° de réf. du vendeur __1936113589

Synopsis :

Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to muscle contraction and nerve impulses. To create spatiotemporally distinct Ca2+ signals, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the various effectors involved.

This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice.

Specific chapters are devoted to Ca2+ signaling techniques in non-mammalian systems, such as plants, yeast, zebrafish, and Xenopus. Methods for assessing Ca2+-binding kinetics and strategies for developing mathematical models of Ca2+ signaling are also included. Thus, this manual is a comprehensive laboratory resource for biochemists, cell and developmental biologists, and physiologists who are using or looking to expand their repertoire of Ca2+ techniques.

À propos des auteurs: University of Leuven

The Babraham Institute

University of Rochester

Détails bibliographiques

Titre : Calcium Techniques: A Laboratory Manual
Éditeur : Cold Spring Harbor Laboratory Pr
Date d'édition : 2013
Reliure : Paperback
Etat : Brand New