Guide to Electroporation and Electrofusion - Couverture rigide

Couverture rigide

ISBN 10 : 0121680401 ISBN 13 : 9780121680404

Editeur : Academic Press Inc, 1992

Synopsis

Guide to Electroporation and Electrofusion is designed to cover all relevant topics pertaining to both electroporation and electrofusion. Divided into four major parts, the book covers fundamental aspects, as well as more advance aspects of the electroporation-electrofusion relationship. The book first covers the basic principles and fundamentals by presenting the most recent theoretical and experimental studies from various fields, such as physics, chemistry, and biology. Next, the book tackles the applications of electroporation and electrofusion in biology, such as transferring, manipulating, and transforming genetic materials. In the third section, the book discusses experimental protocols to serve as a guide when performing experiments using electroporation and electrofusion. The final section discusses the instruments needed to effectively perform an experiment that involves electroporation and electrofusion. This book will be of great used to both novice and advanced researchers whose work involves electroporation and electrofusion, as it provides comprehensive information regarding these topics.

A propos du livre

Présentation de l'éditeur

Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.
Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques.

Key Features
* Covers fundamentals of electroporation and electrofusion in detail
* Molecular events
* Mechanisms
* Kinetics
* Gives extensive practical information
* The latest applications
* Controlling parameters to maximize efficiency
* Available instrumentation
* Presents applications of electroporation and electrofusion in current research situations
* State-of-the-art modifications to electrical pulses and generators
* Application of electroporation and electrofusion to unique, alternative cell and tissue types
* Gives straightforward, detailed, easy-to-follow protocols for
* Formation of human hybridomas
* Introduction of genetic material into plant cells and pollen
* Transfection of mammalian cells
* Transformation of bacteria, plants, and yeast
* Production of altered embryos
* Optimization of electroporation by using reporter genes
* Comprehensive and up-to-date
* Convenient bench-top format
* Approximately 125 illustrations complement the text
* Complete references with article titles
* Written by leading authorities in electroporation and electrofusion

Revue de presse

"The protocols are well presented... Excellent instructions for optimizing the procedures on different cell types... Anyone who wants to use electroporation or electrofusion in anything but a routine fashion will find this book very useful." --ANALYTICAL BIOCHEMISTRY

Détails de l'édition

• Éditeur: Academic Press Inc
• Date d'édition: 1992
• Langue: anglais
• ISBN 10: 0121680401
• ISBN 13: 9780121680404
• Reliure: Relié
• Nombre de pages: 592
• Éditeur: Chang Donald
• Coordonnées du fabricant: non disponible
• Personne responsable: non disponible