Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.
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Vendeur : About Books, Henderson, NV, Etats-Unis
Hardcover. Etat : Near Fine condition. Etat de la jaquette : No Jacket. First Printing of the First Edition. New York:: Academic Press, 1983. A bright, clean copy, but for a small name lined-through on the front endpaper. Square and tight. No remainder mark. Pages are fresh and crisp -- probably never read. No underlining. No highlighting. No margin notes. Illustrated with many diagrams, drawings, schematics, DNA sequences, and autoradiograms. Bibliographical references. Index. Bound in the original blue-green cloth, lettered in shiny silver on the spine. Contains 14 scholarly papers and 2 appendices, including: MULTIPURPOSE EXPRESSION CLONING VEHICLES IN ESCHERICHIA COLI by Yoshihiro Masui, et. al.; VECTORS FOR HIGH-LEVEL, INDUCIBLE EXPRESSION OF CLONED GENES IN YEAST by James R. Broach and Makkuni Jayaram; TWO-DIMENSIONAL DNA ELECTROPHORETIC METHODS UTILIZING IN SITU ENZYMATIC DIGESTIONS by Thomas Yee and Masayori Inouye; etc. . First Printing of the First Edition. Hard Cover. Near Fine condition./No Jacket. 8vo. xiii, 315pp. . N° de réf. du vendeur 005457
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Vendeur : Ammareal, Morangis, France
Hardcover. Etat : Bon. Ancien livre de bibliothèque avec équipements. Couverture différente. Edition 1983. Ammareal reverse jusqu'à 15% du prix net de cet article à des organisations caritatives. ENGLISH DESCRIPTION Book Condition: Used, Good. Former library book. Different cover. Edition 1983. Ammareal gives back up to 15% of this item's net price to charity organizations. N° de réf. du vendeur H-088-425
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Vendeur : Buchpark, Trebbin, Allemagne
Etat : Sehr gut. Zustand: Sehr gut | Seiten: 315 | Sprache: Englisch | Produktart: Bücher | Keine Beschreibung verfügbar. N° de réf. du vendeur 37905587/202
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