Plant Microtechnique And Microscopy - Couverture souple

Ruzin, Steven-E

 
9780195089561: Plant Microtechnique And Microscopy

Synopsis

This text provides in-depth coverage of the field of microtechnique. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background included to give students the tools required to design their own experiments and interpret existing results. In addition, the theory and practice of modern applications, such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in-situ hybridization, are documented thoroughly. Preceding the first chapter are ten single-page Quick Start protocols. Patterned after computer software manuals, these abbreviated instructions provide the experienced student with the fundamental steps to complete the ten microtechnique protocols most frequently used today. Appendices on laboratory practice (chemical toxicities, common calculations and buffer tables) are provided.

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Présentation de l'éditeur

Plant Microtechnique and Microscopy surpases all previously written manuals by providing an in-depth coverage of the field of microtechnique. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background included to give students the tools required to design their own experiments and interpret existing results. In addition, the theory and practice of modern applications, such as methacrylate embedding and sectioning, microwave tissue processing, flourescence histochemistry, and in situ hybridization, are documented thoroughly. Preceding Chapter One are ten single-page "Quick Start" protocols. Patterned after computer software manuals, these abbreviated instructions provide the experienced student with the fundamental steps to complete the ten microtechnique protocols most frequently used today. Appendices on laboratory practice (chemical toxicites, common calculations, and buffer tables) are provided to make this volume a valuable addition to every biological laboratory. Plant Microtechnique and Microscopy contains a definitive chapter on microscopy and, with diagrams and text, describes the optical principles of techniques such as phase contrast, DIC, confocal and deconvolution wide-field microscopy. Also included is an extensive appendix on optics and its application to the microscope. The book's bibliography of over 650 references is a substantial resource for any student of histology, histological technique, or microscopy.

Revue de presse

"I am grateful to Steve Ruzin for assembling this incredibly useful collection of information in such a convenient and handy form." (Plant Systematics & Evolution)

"an archive of expertise in the histochemistry field" (Cell Biology International)

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Autres éditions populaires du même titre

9780195089554: Plant Microtechnique and Microscopy

Edition présentée

ISBN 10 :  0195089553 ISBN 13 :  9780195089554
Editeur : Oxford University Press, 2099
Couverture rigide