Radioisotopes have a wide variety of uses in biological investigations. This book presents practical protocols for application in several types of investigation, including in vivo and in vitro labelling, autoradiography and radioimmunoassay. Also included are guidelines to isotope and equipment choice, safety precautions, radiation protection advice and summaries of legislation covering radioactive material in both the USA and the UK. The use of radioactive material in biological research continues to expand, and this book provides practical protocols on how to choose, handle and make best use of it.
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The value of radioisotopes to biological research is undisputed. Without their availability and use we would be ignorant of a vast range of physiological and biochemical processes, including intermediatary metabolism, neurochemistry, hormone action and protein synthesis. The range and detail of applications is vast and beyond the scope of any book. However, there are general principles and techniques common to all or most radiotracer experiments: in the first place, the decisions to use then chose a radioisotope; secondly, the establishment of a suitable working environment conducive to safe laboratory practice and the compliance with relevant legislation; thirdly, the principles of safe and efficient experimental design, and finally the radiation detection and data analysis. These features, essential to any radioisotope experiment, are fully explored in this book. explores these
'this must be considered as another useful volume in the series ... It contains much in the way of basic methodology simply described and will be helpful for newcomers.' K.R.F. Elliott, FEBS Letters, Volume 279, number 1, February 1991
'This volume is a most welcome addition to this series.' W. Fitschen, Biochemical Education 19(2) 1991
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