Like most scientific disciplines, the techniques for studying genes and DNA are complicated and difficult for the novice to understand. This book provides an introduction to practical molecular biology and is aimed specifically at students and other research workers who have no prior experience of the subject. In this part of the volume the more specialized procedures for gene identification and characterization are covered, with the emphasis on sound technical instruction, together with theoretical background and trouble-shooting guidance. The aim is to give the reader a sufficiently strong knowledge of these general techniques to allow the procedures to be used with confidence in specific research applications.
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The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements. Volume 1 concentrates on the procedures for DNA and RNA manipulation: purification, electrophoresis, and the construction and cloning of recombinant molecules. It also includes a general introduction to molecular biology in the laboratory and a survey of cloning vectors for Escherichia Coli.
Brown becomes the Delia Smith of molecular biology, starting with how to boil an egg, before proceeding to more complex recipes (Journal of Cell Science )
Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.
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