R.N.A. Processing: A Practical Approach, Volume 1 (Paper) - Couverture souple

Couverture souple

ISBN 10 : 0199633436 ISBN 13 : 9780199633432

Editeur : Oxford University Press, 1998

Synopsis

As post-translational events become increasingly recognized as critical regulation sites of gene expression in eukaryotic cells, intense research interest in R.N.A. processing has rapidly grown. R.N.A. Processing: A Practical Approach, available in two volumes, offers detailed guidance on all major aspects of the subject. Step-by-step protocols from leading laboratories are presented for studying the termination, end-processing, capping, methylation, splicing, and editing of M.R.N.A. . Protocols designed for the study of M.R.N.A. stability and processing of R.R.N.A. and T.R.N.A. are included as well, with full descriptions of the synthesis and purification of R.N.A. substrate for in vitro work, the characterization of specific R.N.A.s, and the isolation and analysis of ribonucleoprotein complexes. Taken together, the two volumes are invaluable laboratory companions to researchers working in M.R.N.A., T.R.N.A., and T.R.N.A. expression. The contributors' lucid explanatory style and comprehensive coverage will be welcomed by both experienced researchers and by students embarking on such studies for the first time.

A propos du livre

Présentation de l'éditeur

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end-processing, capping, methylation, splicing, and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all the key steps required for a successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNAs, and the isolation and analysis of ribonucleoprotein complexes. RNA processing - A Practical Approach, Volumes I and II, are invaluable laboratory companions for researchers working on mRNA, rRNA, or tRNA gene expression. Their lucid explanatory style and comprehensive coverage of all the key techniques for analysing RNA processing will be welcomed by both experienced researchers and

Détails de l'édition

• Éditeur: Oxford University Press
• Date d'édition: 1998
• Langue: anglais
• ISBN 10: 0199633436
• ISBN 13: 9780199633432
• Reliure: Broché
• Nombre de pages: 232
• Éditeur: Higgins Stephen J.
• Coordonnées du fabricant: non disponible
• Personne responsable: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8; Assago Milano; 20090; Italie