Affinity Separations: A Practical Approach - Couverture rigide

Matejtschuk, Paul

Couverture rigide

ISBN 10 : 019963551X ISBN 13 : 9780199635511

Editeur : Oxford University Press, 1997

Synopsis

This is a collection of affinity-based purification protocols with an international authorship from across the life sciences. The introductory chapter outlines recent advances in ligand-coupling, matrix choice, and operating modes. The second chapter describes methods for obtaining quantitative data from affinity processes. There are specific chapters describing methods for the affinity separation of proteins, nucleic acids, and oligosaccharides. Methods for immunoglobulin purification and immunoaffinity protocols are discussed in a further chapter, and the use of affinity tags in the recovery of engineered proteins in another. Finally, modern techniques for cell separations are outlined. The book presents detailed laboratory protocols together with troubleshooting advice and reviews of recent developments. This book is aimed at bench scientists, both those in analytical research laboratories, and those non-specialists looking to apply affinity methods in their work; postgraduate and post-doctoral researchers, and academic staff in analytical research and general bioscience laboratories.

A propos du livre

Présentation de l'éditeur

Affinity Separations: A Practical Approach is a new collection of affinity-based purification protocols with an international authorship from across the life sciences. The introductory chapter outlines recent advances in ligand-coupling, matrix choice, and operating modes. The second chapter describes methods for obtaining quantitative data from affinity processes. There are specific chapters describing methods for the affinity separation of proteins, nucleic acids, and oligosaccharides. Methods for immunoglobulin purification and immunoaffinity protocols are discussed in a further chapter, and the use of affinity tags in the recovery of engineered proteins in another. Finally, modern techniques for cell separations are outlined. The book presents detailed laboratory protocols together with troubleshooting advice and reviews of recent developments. It is aimed at bench scientists, both those in analytical research laboratories, and those non-specialists looking to apply affinity methods in their work.

Détails de l'édition

• Éditeur: Oxford University Press
• Date d'édition: 1997
• Langue: anglais
• ISBN 10: 019963551X
• ISBN 13: 9780199635511
• Reliure: Relié
• Nombre de pages: 272
• Coordonnées du fabricant: non disponible
• Personne responsable: Messaggerie Libri; ufficio.anagrafiche@meli.it; ; Via Verdi, 8; Assago Milano; Italie