High Resolution Chromatography: A Practical Approach - Couverture rigide

 
9780199636495: High Resolution Chromatography: A Practical Approach
Couverture rigide
ISBN 10 : 0199636494 ISBN 13 : 9780199636495
Editeur : Oxford University Press, 1999

Synopsis

The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.

Revue de presse

"Laboratory workers will find this a useful guide to the development of effective purification protocols" Aslib Book Guide"

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
Oxford University Press
Date d'édition
1999
Langue
anglais
ISBN 10 
0199636494
ISBN 13 
9780199636495
Reliure
Relié
Nombre de pages
333
Éditeur
Millner Paul
Coordonnées du fabricant
non disponible
Personne responsable
Ufficio Anagrafiche Messaggerie
ufficio.anagrafiche@meli.it

Via Verdi, 8
Assago Milano
20090
Italie

Autres éditions populaires du même titre

9780199636488: High Resolution Chromatography: A Practical Approach

Edition présentée

ISBN 10 :  0199636486 ISBN 13 :  9780199636488
Editeur : Oxford University Press, USA, 1999
Couverture souple