Proteomics Mass Spectrometry Methods - Couverture souple

Couverture souple

ISBN 10 : 0323903959 ISBN 13 : 9780323903950

Editeur : Academic Press, 2024

Synopsis

Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols shares best practices collected across key laboratories and core facilities, taking the reader through key tactics for executing the most usual mass spectrometry experiments. Sections review research making use of MS proteomics experiments, focus on critical sample preparation, cover mammalian cell lines and samples from clinical tissue and biological fluids, discuss subcellular fractionation, provide methods for protein digestion both for in gel and in solution, and delve into key MS proteomics analysis protocols, including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiquination enrichment, and more. This book is the perfect lab manual for research teams or for use as a new staff training material. Core facility managers may also find it useful for sharing best practices with their staff and researchers.

A propos du livre

À propos de l'auteur

Dr. Meleady received her degree in Biotechnology from the Dublin City University and PhD from the National Cell and Tissue Culture Centre. She is currently Senior Research Scientist, Programme Leader, Proteomics Core Facility, and Associate Director National Institute for Cell Biology.

Détails de l'édition

• Éditeur: Academic Press
• Date d'édition: 2024
• Langue: anglais
• ISBN 10: 0323903959
• ISBN 13: 9780323903950
• Reliure: Broché
• Nombre de pages: 240
• Éditeur: Meleady
• Coordonnées du fabricant: non disponible
• Personne responsable: Barbara Criscuolo; b.criscuolo@lswr.it; ; Via Spadolini, 7; Milano; 20141; Italie