How to Genotype Rotavirus Isolates - Couverture souple

Anochie, Philip

Couverture souple

ISBN 10 : 0359002056 ISBN 13 : 9780359002054

Editeur : Lulu.com, 2018

Synopsis

Viral RNA was extracted from the stool samples collected from the children and analyzed using one step Reverse Transcriptase Polymerase Chain Reaction (RT-PCR ) for genotyping and Agarose Gel Electrophoresis (AGE) for the identification of the different bands of the electrophoretypes of the rotavirus. Analysis results indicated that 16 (22.53%%) out of the 71 children were positive for rotavirus infection while 55 (77.46%%) were negative for rotavirus infection. The rotavirus positive patients were made up of 12 males (68.75%%) and 4 females (25.0%%) while rotavirus negative patients were 32 males (58.18%%) and 23 females (41.81%%). The total number of males with gastroenteritis were 44 (60.56%%) while females were 27 (39.43%%). Rotavirus G2 genotype detected in 10 patients was the most prevalent strain in the hospital followed by G1 genotype found in 6 patients and these were the only rotavirus genotypes detected in the hospital.

Détails de l'édition

• Éditeur: Lulu.com
• Date d'édition: 2018
• Langue: anglais
• ISBN 10: 0359002056
• ISBN 13: 9780359002054
• Reliure: Broché
• Nombre de pages: 124
• Coordonnées du fabricant: RUE DE SEVRES; https://www.hachette.fr/; ; 1 AVENUE GUTENBERG; MAUREPAS CEDEX; 78319; France