Enzymes in RNA Science and Biotechnology, Volume 691 in the Methods in Enzymology series, highlights new advances in the field, including chapters on Reverse transcriptase Part I (discovery, preparation, general utilization, MarathonRT for routine RT-PCR and for cDNA synthesis on challenging RNA templates Structured RNAs, repeat RNAs and more, Engineering TNA Polymerases Through Iterative Cycles of Directed Evolution, Reverse transcriptase Part II (RNA structure mapping and determination), RNA G-quadruplex (rG4) structure detection using RTS and SHALiPE assays, tRNA Structure-seq in vivo and in droplets, Capture the in vivo intact RNA structurome by CAP-STRUCTURE-seq, and more.
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Dr. Chun Kit Kwok obtained his B.Sc. in Chemistry (2009) from the Chinese University of Hong Kong, after completing an exchange program at University of California, Los Angeles in 2007-2008. He completed his PhD in Pennsylvania State University (2014), mentored by Professor Philip C. Bevilacqua and Professor Sarah M. Assmann. In Apr 2014, Dr. Kwok worked as a Croucher Postdoctoral Fellow in University of Cambridge under Professor Sir Shankar Balasubramanian. In Oct 2016, Dr. Kwok's joined the City University of Hong Kong as an Assistant professor and has been promoted to Associate professor in July 2021. In 2019, he was one of the recipients of the CityU President Award and Croucher Innovation Award. In 2020, Dr. Kwok's joined the State Key Laboratory of Marine Pollution, City University of Hong Kong. In 2021, Dr. Kwok was recognized as elected member of Hong Kong Young Academy of Science (YASHK) and Hong Kong Institute for Advanced Study Rising Star in Chemistry. In 2022, Dr. Kwok was awarded the NSFC Excellent Young Scientist Fund.
Dr. Kwok's current research focus is to explore the role of RNA structures and interactions in biology, especially the functions of G-quadruplex structures/interactions and non-coding RNA structures/interactions in the mammalian transcriptome and their relevance to gene regulation, RNA metabolism and diseases. Two new research directions in the Kwok lab are to develop targeting tools for detection, imaging, intervention of these important RNA structures and interactions, as well as to invent innovative nucleic acid-based technologies for sensing chemical pollutants and pathogensLes informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.
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