Gene Discovery for Disease Models - Couverture rigide

Couverture rigide

ISBN 10 : 047049946X ISBN 13 : 9780470499467

Editeur : John Wiley & Sons Inc, 2011

Synopsis

This book provides readers with new paradigms on the mutation discovery in the post-genome era. The completion of human and other genome sequencing, along with other new technologies, such as mutation analysis and microarray, has dramatically accelerated the progress in positional cloning of genes from mutated models. In 2002, the Mouse Genome Sequencing Consortium stated that "The availability of an annotated mouse genome sequence now provides the most efficient tool yet in the gene hunter's toolkit. One can move directly from genetic mapping to identification of candidate genes, and the experimental process is reduced to PCR amplification and sequencing of exons and other conserved elements in the candidate interval. With this streamlined protocol, it is anticipated that many decades-old mouse mutants will be understood precisely at the DNA level in the near future." The implication of such a statement should be similar to the identification of mutated genes from human diseases and animal models, when genome sequencing is completed for them. More than five years have passed, but genes in many human diseases and animal models have not yet been identified. In some cases, the identification of the mutated genes has been a bottleneck, because the genetic mechanism holds the key to understand the basis of the diseases. However, an integrative strategy, which is a combination of genetic mapping, genome resources, bioinformatics tools, and high throughput technologies, has been developed and tested. The classic paradigm of positional cloning has evolved with completely new concepts of genomic cloning and protocols. This book describes new concepts of gene discovery in the post-genome era and the use of streamlined protocols to identify genes of interest. This book helps identify not only large insertions/deletions but also single nucleotide mutations or polymorphisms that regulate quantitative trait loci (QTL).

A propos du livre

À propos de l?auteur

Weikuan Gu received his PhD from Cornell University in 1994 and joined the University of Tennessee Health Science Center as an assistant professor in 2002. Dr. Gu's lab has developed an integrated strategy for the positional cloning of genes, a strategy which has been successfully applied to clone several genes from spontaneous mouse mutations.

Yongjun Wang received his MD from Hebei Medical College in 1982 and his MBA from Peking University in 2004. Dr. Wang joined Beijing Tiantan Hospital in 2000 and now serves as the vice president of the hospital.

Détails de l'édition

• Éditeur: John Wiley & Sons Inc
• Date d'édition: 2011
• Langue: anglais
• ISBN 10: 047049946X
• ISBN 13: 9780470499467
• Reliure: Relié
• Nombre de pages: 560
• Éditeur: Gu Weikuan, Wang Yongjun
• Coordonnées du fabricant: non disponible
• Personne responsable: non disponible