Articles liés à Green Fluorescent Protein: Properties, Applications,...
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Synopsis
This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
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Quatrième de couverture
Edited by Martin Chalfie and Steven Kain
Green fluorescent protein (GFP), a biological marker cloned from jellyfish, has emerged from virtual obscurity into the forefront of biomolecular research in just a few years. Intensely studied in hundreds of in vivo projects worldwide, and resulting in new articles and applications at a breathtaking rate, GFP may well become one of the most powerful tools in molecular and cellular biology.
Green Fluorescent Protein: Properties, Applications, and Protocols is the first book devoted to this rapidly evolving reporter system. This collection of essays from pioneers in the field tackles both theory and practice, offering numerous case studies, examples, illustrations, and troubleshooting tips. The book clearly demonstrates how to tailor GFP to specific systems, maximize expression and enhance detection in a variety of organisms and cell types, generate variants with altered properties, explore new ways to use GFP, and more. Topics covered include:
∗ The discovery of GFP
∗ Bioluminescence and biofluorescence in nature
∗ Biochemical and physical properties of GFP
∗ The three–dimensional structure of GFP and its implications for function and design
∗ Molecular biology and modification of GFP
∗ Uses of GFP in various organisms, including prokaryotes, yeast, C. elegans, drosophila, plants, transgenic vertebrates, and mammalian cells
∗ Detailed protocols for the expression of GFP, specimen preparation, visualization and recording of GFP fluorescence, and GFP websites.
Green Fluorescent Protein: Properties, Applications, and Protocols lays the foundation for a new understanding of dynamic biological phenomena. It is essential reading for researchers and graduate students in molecular biology, physiology, biochemistry, cell and developmental biology, neuroscience, ecology, in vivo biology, and plant molecular biology.
Biographie de l'auteur
MARTIN CHALFIE, PhD , is William R. Kenan Jr. Professor of Biological Sciences at Columbia University. He is world renowned for introducing the use of GFP as a biological marker. A member of the National Academy of Sciences, Dr. Chalfie serves on the editorial boards of Genome Biology and Molecular Biology of the Cell. STEVEN R. KAIN, PhD , manages the Applications Gene Expression Group for Agilent Technologies. While director of cell biology at Clontech Laboratories, he was responsible for commercializing the first products based on GFP and produced the popular EGFP variant. He has twelve years′ experience with a variety of biotechnology firms in the San Francisco Bay Area.
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