This tutorial explores flow cytometry and its applications in research and diagnostic laboratory settings. Written for the interested novice and non-specialist, it outlines basic principles of instrumentation, sample preparation, analysis and applications in the life and biomedical sciences.
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Flow cytometry continually amazes scientists with its ever–expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis.
Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state–of–the–art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high–speed sorting and alternative sorting methods, as well as traditional technology. This essential resource:
Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
"...reflects innovations in the field since the first edition was published..." ( SciTech Book News, Vol. 26, No. 2, June 2002)
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