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High-performance liquid chromatography (HPLC) has long been recognized as a useful and versatile analytical technique. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently, there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of its principles and practice. This is an introduction to the fundamentals of HPLC, written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines. The text is logically structured, beginning with an introduction, which then progresses to the essential knowledge of the instrumentation needed for HPLC and its applications.
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