Handbook of Molecular and Cellular Methods in Biology and Medicine - Couverture souple

Kaufman, Peter B.

Couverture souple

ISBN 10 : 0849325110 ISBN 13 : 9780849325113

Editeur : CRC Press Inc, 1995

Synopsis

"Reflecting the milestones in the field that have occurred since the publication of the first edition, this book provides an updated tool kit that incorporates conventional as well as modern approaches to tackling biological and medicinal research in thepost genomic era. The authors have evaluated, revised, and sometimes replaced all protocols with more efficient, reliable, or simpler ones. The book is also completely reorganized and covers topics of modern "omics" approaches, including genomics, proteomics, and metabolomics. This is followed coverage of the Manipulation of Biological Systems, including RNAi, and the use of microscopy"--Provided by publisher.

A propos du livre

Présentation de l'éditeur

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method.

This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies.

The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Détails de l'édition

• Éditeur: CRC Press Inc
• Date d'édition: 1995
• Langue: anglais
• ISBN 10: 0849325110
• ISBN 13: 9780849325113
• Reliure: Broché
• Nombre de pages: 496
• Éditeur: Kim Donghern
• Coordonnées du fabricant: non disponible
• Personne responsable: non disponible