Basic Methods in Protein Purification and Analysis - Couverture souple

 
9780879698676: Basic Methods in Protein Purification and Analysis
Couverture souple
ISBN 10 : 0879698675 ISBN 13 : 9780879698676
Editeur : Cold Spring Harbor Laboratory Press,U.S., 2008

Synopsis

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. Contents: 1. Introduction to Protein Purification Strategies 2. Preparation of Cellular and Subcellular Extracts Section 1: Purification Using Gel Electrophoresis 3. One-dimensional Polyacrylamide Gel Electrophoresis 4. Preparative Two-dimensional Gel Electrophoresis with Immobilized pH Gradients Section 2: Purification Using Column Chromatography 5. Ion-exchange Chromatography 6. Size-exclusion Chromatography 7. Reversed-phase High-performance Liquid Chromatography 8. Affinity and Immunoaffinity Chromatography 9. Metal Chelate Affinity Chromatography 10. Multidimensional Chromatography of Intact Proteins Section 3: Characterizing Protein Complexes 11. Identification of Protein Protein Interactions by Conventional Column Chromatography 12. Identification of Associated Proteins by Coimmunoprecipitation 13. Chromatin Immunoprecipitation of Protein Complexes 14. Identification of Protein Protein Interactions with Glutathione-S-Transferase Fusion Proteins 15. Direct Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation Analysis 16. In silico Tools: Analysis for Creating Focused Interaction Networks Appendices 1. Measuring the Concentration of Proteins 2. Concentrating Solutions of Proteins 3. Stabilization of Proteins for Storage 4. Immunoblotting and Staining of Membrane-bound Proteins 5. General Cautions Index

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity of a protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein protein interactions. The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts a critical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing protein protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. Contents: 1. Introduction to Protein Purification Strategies 2. Preparation of Cellular and Subcellular Extracts Section 1: Purification Using Gel Electrophoresis 3. One-dimensional Polyacrylamide Gel Electrophoresis 4. Preparative Two-dimensional Gel Electrophoresis with Immobilized pH Gradients Section 2: Purification Using Column Chromatography 5. Ion-exchange Chromatography 6. Size-exclusion Chromatography 7. Reversed-phase High-performance Liquid Chromatography 8. Affinity and Immunoaffinity Chromatography 9. Metal Chelate Affinity Chromatography 10. Multidimensional Chromatography of Intact Proteins Section 3: Characterizing Protein Complexes 11. Identification of Protein Protein Interactions by Conventional Column Chromatography 12. Identification of Associated Proteins by Coimmunoprecipitation 13. Chromatin Immunoprecipitation of Protein Complexes 14. Identification of Protein Protein Interactions with Glutathione-S-Transferase Fusion Proteins 15. Direct Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation Analysis 16. In silico Tools: Analysis for Creating Focused Interaction Networks Appendices 1. Measuring the Concentration of Proteins 2. Concentrating Solutions of Proteins 3. Stabilization of Proteins for Storage 4. Immunoblotting and Staining of Membrane-bound Proteins 5. General Cautions Index

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
Cold Spring Harbor Laboratory Press,U.S.
Date d'édition
2008
Langue
anglais
ISBN 10 
0879698675
ISBN 13 
9780879698676
Reliure
Broché
Nombre de pages
436
Éditeur
Simpson Richard J., Adams Peter D., Golemis Erica A.
Coordonnées du fabricant
non disponible
Personne responsable
non disponible

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9780879698683: Basic Methods in Protein Purification and Analysis

Edition présentée

ISBN 10 :  0879698683 ISBN 13 :  9780879698683
Editeur : Cold Spring Harbor Laboratory Pr..., 2008
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