Baculovirus Expression Protocols - Couverture souple

Couverture souple

ISBN 10 : 0896032728 ISBN 13 : 9780896032729

Editeur : Humana Press Inc., 2009

Synopsis

Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors - leading authorities in the field - present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols. It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2009
• Langue: anglais
• ISBN 10: 0896032728
• ISBN 13: 9780896032729
• Reliure: Broché
• Nombre de pages: 436
• Éditeur: Richardson Christopher D.
• Coordonnées du fabricant: non disponible
• Personne responsable: HUMANA SPAIN S.L.; https://www.humana-spain.org/?lang=en; ; Av Alberto Alcocer 46, 5-B; Madrid; 28016; Espagne