Diagnostic Virology Protocols - Couverture souple

Couverture souple

ISBN 10 : 0896034011 ISBN 13 : 9780896034013

Editeur : Humana, 1998

Synopsis

The accurate and reliable diagnosis of transmissible diseases is the most powerful weapon available to ensure their control, and in some cases eradication. The detection of parasites in clinical cases, companion and farm animals, and in the environment is relatively easy since many of them are visible to the naked eye, and those that are not are readily detected by light microscopy. Fungal infections can similarly be determined. Bacteria are somewhat harder to detect. Although their presence can frequently be detected by light microscopy, differential diagnosis, beyond their gross morphology, is almost always impossible. However, most bacterial pathogens can be cultured in the laboratory and can be accurately identified by combinations of a series of simple tests such as morphology, staining, antibiotic sensitivity, biochemical analyses, nutrient dependence, and phage sensitivity. Viruses, however, have proved much more difficult; their size and absolute dependence of the host cell for propagation have rendered useless the methods traditionally used for other microorganisms. Until the development of tissue culture in the middle of this century, diagnosis was entirely dependent on the skill and experience of the clinician. But this was an unreliable process since many of the common virus infections exhibit similar clinical symptoms, such as coryza, exanthema, vomiting, diarrhea, neuralgia, and lethargy. Indeed many viral infections display clinical signs that are indistinguishable from bacterial or parasitic infections.

A propos du livre

Présentation de l'éditeur

This second edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). It also covers robotics as well as future prospects.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 1998
• Langue: anglais
• ISBN 10: 0896034011
• ISBN 13: 9780896034013
• Reliure: Broché
• Nombre de pages: 388
• Éditeur: Stephenson John R., Warnes Alan
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne