Mycobacteria Protocols - Couverture rigide

Parish, T.; Stoker, N.G.

Couverture rigide

ISBN 10 : 0896034712 ISBN 13 : 9780896034716

Editeur : Humana Press Inc., 2010

Synopsis

Laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease, including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis.

A propos du livre

Présentation de l'éditeur

Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need. Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field. Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology TM series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2010
• Langue: anglais
• ISBN 10: 0896034712
• ISBN 13: 9780896034716
• Reliure: Relié
• Nombre de pages: 492
• Coordonnées du fabricant: non disponible
• Personne responsable: HUMANA SPAIN S.L.; https://www.humana-spain.org/?lang=en; ; Av Alberto Alcocer 46, 5-B; Madrid; 28016; Espagne