Pcr Mutation Detection Protocols: Methods in Molecular Biology - Couverture souple

Couverture souple

ISBN 10 : 0896036170 ISBN 13 : 9780896036178

Editeur : Humana Press Inc., 2002

Synopsis

Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

A propos du livre

Présentation de l'éditeur

The Second Edition updates the subject with key protocols involving the PCR and its many incarnations such as SSCP, CSGE, and dHPLC. The book also addresses such areas as Southern blotting, accurate diagnostics with high throughput, and microarray systems.

Détails de l'édition

• Éditeur: Humana Press Inc.
• Date d'édition: 2002
• ISBN 10: 0896036170
• ISBN 13: 9780896036178
• Reliure: Broché
• Langue: anglais
• Nombre de pages: 224
• Éditeur: Theophilus Bimal D.M., Rapley Ralph
• Coordonnées du fabricant: non disponible