Transgenesis Techniques: Principles and Protocols - Couverture rigide

 
9780896036963: Transgenesis Techniques: Principles and Protocols
Couverture rigide
ISBN 10 : 0896036960 ISBN 13 : 9780896036963
Editeur : Humana Press Inc., 2002

Synopsis

This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Éditeur
Humana Press Inc.
Date d'édition
2002
Langue
anglais
ISBN 10 
0896036960
ISBN 13 
9780896036963
Reliure
Relié
Numéro d'édition
2
Nombre de pages
368
Éditeur
Clarke Alan
Coordonnées du fabricant
non disponible
Personne responsable
non disponible