Pcr Cloning Protocols - Couverture rigide

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ISBN 10 : 0896039730 ISBN 13 : 9780896039735

Editeur : Humana Press Inc., 2002

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PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Les informations fournies dans la section � Synopsis � peuvent faire r�f�rence � une autre �dition de ce titre.

�diteur: Humana Press Inc.
Date d'�dition: 2002
Langue: anglais
ISBN 10: 0896039730
ISBN 13: 9780896039735
Reliure: Reli�
Num�ro d'�dition: 2
Nombre de pages: 453
�diteur: Chen Bing-Yuan, Janes Harry W.
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Personne responsable: non disponible

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Editeur : Humana Press Inc., 2002
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PCR Cloning Protocols: From Molecular Cloning to Genetic Engineering (Methods in Molecular Biology)

Edit� par Humana Press, 2002

ISBN 10 : 0896039730 ISBN 13 : 9780896039735

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PCR Cloning Protocols / Hrsg. Bing-Yuan Chen , Hrsg. Harry W. Janes

Chen, Bing-Yuan (Herausgeber)Janes, Harry W. (Herausgeber)

Edit� par Totowa : Humana Press, 2002

ISBN 10 : 0896039730 ISBN 13 : 9780896039735

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Etat : Gut. Ausgabe: 2., Ed., 2002 Umfang/Format: 460 Seiten , 229 mm x 152 mm Einbandart und Originalverkaufspreis: : EUR 85.55 (unverbindliche Preisempfehlung), sfr 131.00 (unverbindliche Preisempfehlung) 0-89603-973-0 : EUR 85.55 (unverbindliche Preisempfehlung), sfr 131.00 (unverbindliche Preisempfehlung) PCR Cloning Protocols, Second Edition, updates and expands Bruce White, s best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA f Rutgers University, New. Brunswick From Reviews of the First Edition: .a balanced and easy to use guide to the principal contemporary options. Microbiology Today .eliminatles] mang laborious and expensive techniques associated with traditional Isolation and analysis. Biochemical Education In the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes hy mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White, s best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis an long-distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination and to clone the challenging uncharacterized DNA flanking a known DNA fragment. Powerful applications of PCR in library construction and sublibrary generation and screening are also presented. Authoritative and up-to-date, PCR Cloning Protocols, Second Edition, constitutes a gold-standard collection of the fastest, simplest, and most popular methods for isolating genes from all biological samples and creating novel genes from them by mutagenesis/recombination essential methods for today, s study of functional genomics, gene expression, protein structure function relationships, protein engineering, and molecular evolution. Readily reproducible methods for isolating genes from all biological samples Cutting-edge techniques described by hands-on masters Azffiffleses Proven methods for cloning uncharacterized DNA z- flanking a known DNA fragment 111 Tested techniques for in vitro DNA recombination Part I. Performing and Optimizing PCR. Part II. Clon- bination. Part IV. Cloning Unknown Neighboring ing PCR Products. Part III. Mutagenesis and Recom- DNA. Part V. Library Construction and Screening. gutes Exemplar, ordentlich, Gern k�nnen sie Ihr Buch per Rechnung bestellen. Hardcover. N� de r�f. du vendeur B00070424

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PCR Cloning Protocols

Harry W. Janes Bmg-Yuan Chen

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PCR Cloning Protocols

Janes Harry W. Chen Bmg-Yuan

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PCR Cloning Protocols

Janes Harry W. Chen Bmg-Yuan

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Pcr Cloning Protocols 2 Ed (Pb)

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Pcr Cloning Protocols 2 Ed (Pb)

Chen Y.B.

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PCR CLONING PROTOCOLS 2 ED (PB)

CHEN Y.B.

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PCR Cloning Protocols (Methods in Molecular Biology)

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