CRISPR Guide RNA Design: Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1071606891 ISBN 13 : 9781071606896

Editeur : Humana, 2021

Synopsis

This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2021
• Langue: anglais
• ISBN 10: 1071606891
• ISBN 13: 9781071606896
• Reliure: Broché
• Nombre de pages: 296
• Éditeur: Fulga Tudor A., Knapp David J. H. F., Ferry Quentin R. V.
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3,69115 Heidelberg, Germany; Heidelberg; 69115; Allemagne