Part I: Introductory Reviews
1. Introduction and Classification of Leukemias
Geoffrey Brown
2. Leukemia Stem Cells: Concept and Implications
Isidro Sánchez-García and César Cobaleda
3. Leukemia Stem Cell Drug Discovery
César Cobaleda and Isidro Sánchez-García
Part II: Protocols
4. Analysis and Isolation of Mouse Leukemic Stem Cells
Fang Dong, Haitao Bai, and Hideo Ema
5. Mass Cytometry of Hematopoietic Cells
Astraea Jager, Jolanda Sarno, and Kara Davis
6. PCR Technology to Identify Minimal Residual Disease
Giovanni Cazzaniga, Simona Songia, and Andrea Biondi, on behalf of the EuroMRD Working Group
7. Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies
Michaela Kotrova, Nikos Darzentas, Christiane Pott, and Monika Brüggemann, on behalf of the EuroClonality-NGS Working Group
8. Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)
Daniel Hein, Arndt Borkhardt, and Ute Fischer
9. Single-Cell Transcriptomic Analysis of Hematopoietic Cells
Paulina M. Strzelecka, Anna M. Ranzoni, and Ana Cvejic
10. In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics
Yiran Liang, Thy Truong, Ying Zhu, and Ryan T. Kelly
11. In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation
Kyomi J. Igarashi and Ryo Yamamoto
12. Experimental Competitive Bone Marrow Transplant Assays
Roxann Hétu-Arbour, Sarah Bouali, and Krista M. Heinonen
13. Human T-ALL Xenografts
Patricia Fuentes, María L. Toribio, and Sara González-García
14. An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts
Thalia Vlachou, Marco S. Nobile, Chiara Ronchini, Daniela Besozzi, and Pier Giuseppe Pelicci
15. Leukemic Stem Cell Culture in Cytokine-Free Medium
Xiaolei Liu and Peter S. Klein
16. Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid
Luena Papa, Mansour Djedaini, Manisha Kintali, Christoph Schaniel, and Ronald Hoffman
17. Isolation, Culture, and Manipulation of Human Cord Blood Progenitors
Cristina Priet
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Gebunden. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsIncludes cutting-edge techniquesProvides step. N° de réf. du vendeur 364129892
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Leukemia Stem Cells: Methods and Protocols serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research. 496 pp. Englisch. N° de réf. du vendeur 9781071608098
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