Synopsis
Part I: Introduction
1. Modified Forms of Cytosine in Eukaryotes: DNA (De)Methylation and Beyond
Maria Eleftheriou and Alexey Ruzov
2. Evidence for Non-Cytosine Epigenetic DNA Modifications in Multicellular Eukaryotes: An Overview
Paige Lowe, Ryszard Olinski, and Alexey Ruzov
3. Mass Spectrometry-Based Analysis of DNA Modifications: Potential Applications in Basic Research and Clinic
Ryszard Olinski, Rafal Rozalski, and Daniel Gackowski
4. Mapping DNA Methylation in Mammals: The State of the Art
Antonio Lentini and Colm E. Nestor
Part II: Detection and Quantification of DNA Modifications Using Mass Spectrometry and Capillary Gel Electrophoresis
5. Detection of Modified Bases in Bacteriophage Genomic DNA
Yan-Jiun Lee and Peter R. Weigele
6. Liquid Chromatography-Mass Spectrometry Analysis of Cytosine Modifications
Nan Dai and Ivan R. Corrêa Jr.
7. Detection of DNA Methylation in Genomic DNA by UHPLC-MS/MS
Konstantinos Boulias and Eric Lieberman Greer
8. Quantification of DNA Modifications Using Two-Dimensional Ultra-Performance Liquid Chromatography Tandem Mass-Spectrometry (2D-UPLC-MS/MS)
Marta Starczak, Maciej Gawronski, Ryszard Olinski, and Daniel Gackowski
9. MS Analysis of DNA Modifications in Urinary/Body Fluids
Aleksandra Skalska, Agnieszka Siomek-Gorecka, Ryszard Olinski, and Rafal Rozalski
10. Preparation of Internal Standards for 2D-UPLC-MS/MS Quantification of Non-Canonical DNA Bases
Marta Starczak, Aleksandra Skalska, Rafal Rozalski, Ryszard Olinski, and Daniel Gackowski
11. Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis
Romualdas Vaisvila, Lise Hunault, and Lana Saleh
Part III: Assessing the Levels and Spatial Distribution of DNA Modifications Using Antibody-Based Approaches
12. Immunochemical Detection of Modified Cytosine Species in Mammalian Preimplantation Embryos
Tie-Bo Zeng and Piroska E. Szabó
13. Immunochemical Detection of Modified Cytosine Species in Lampbrush Chromatin
Garry T. Morgan
14. Detection of Low-Abundance DNA Modifications Using Signal Amplification-Based Immunocytochemistry
Abdulkadir Abakir and Alexey Ruzov
15. Immunohistochemical Detection of Modified Cytosine Bases in Rodent Brain
Marcus D. Wallis and Rebecca C. Trueman
16. Immunohistochemical Detection of 5-Hydroxymethylcytosine and 5-Ca
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DNA Modifications: Methods and Protocols (Methods in Molecular Biology, 2198)
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techniques for the detection and quantification of DNA modifications, antibody-based approaches to study their spatial distribution in different cells and tissues, and methods to analyze their genomic distribution with the help of bioinformatics tools that interrogate the corresponding datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, DNA Modifications: Methods and Protocols serves as an ideal guide to research scientists and PhD students in this rapidly developing discipline, and, thus, will ultimately contribute to deciphering the roles of non-canonical DNA nucleotides in different biological systems. 512 pp. Englisch. N° de réf. du vendeur 9781071608753
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Buch. Etat : Neu. Neuware -This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techniques for the detection and quantification of DNA modifications, antibody-based approaches to study their spatial distribution in different cells and tissues, and methods to analyze their genomic distribution with the help of bioinformatics tools that interrogate the corresponding datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 512 pp. Englisch. N° de réf. du vendeur 9781071608753
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techniques for the detection and quantification of DNA modifications, antibody-based approaches to study their spatial distribution in different cells and tissues, and methods to analyze their genomic distribution with the help of bioinformatics tools that interrogate the corresponding datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, DNA Modifications: Methods and Protocols serves as an ideal guide to research scientists and PhD students in this rapidly developing discipline, and, thus, will ultimately contribute to deciphering the roles of non-canonical DNA nucleotides in different biological systems. N° de réf. du vendeur 9781071608753
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