Coronaviruses: Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1071609025 ISBN 13 : 9781071609026

Editeur : Humana, 2021

Synopsis

1. Coronaviruses: An Updated Overview of Their Replication and Pathogenesis

Yuhang Wang, Matthew Grunewald, and Stanley Perlman

Part I: Coronavirus Detection, Discovery, and Evolution

2. Discovery of Novel Coronaviruses in Rodents

Theocharis Tsoleridis and Jonathan K. Ball

3. Detection and Discovery of Coronaviruses in Wild Bird Populations

Chantal J. Snoeck and Siamak Zohari

4. Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV)

Shuetsu Fukushi

5. Whole Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing

Graham L. Freimanis and Michael S. Oade

Part II: Propagation and Titration of Coronaviruses

6. Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study

Hui Hu, Kwonil Jung, Scott P. Kenney, and Linda J. Saif

7. The Preparation of Chicken Kidney Cell Cultures for Virus Propagation

Elena Lokhman, Srijana Rai, and Billy Matthews

8. The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation

Giulia Dowgier and Erica Bickerton

9. Isolation and Propagation of Coronaviruses in Embryonated Eggs

James S. Guy

10. Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures

Mitra Gultom, Laura Laloli, and Ronald Dijkman

11. Quantification of Coronaviruses by Titration In Vitro and Ex Vivo

Giulia Dowgier and Helena J. Maier

Part III: Manipulating the Genomes of Coronaviruses

12. Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses

Sarah Keep, Paul Britton, and Erica Bickerton

13. In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline

Tran Thi Nhu Thao, Fabien Labroussaa, Nadine Ebert, Joerg Jores, and Volker Thiel

Part IV: Studying Virus-Host Interactions

14. Proximity Labeling for Identification of Coronavirus-Host Protein Interactions

Philip V'kovski, Silvio Steiner, and Volker Thiel

15. Using Yeast to Identify Coronavirus-Host Protein Interactions

Stuart Weston and Matthew Frieman

16. Determining How Coronaviruses Overcome the Interferon and Innate Immune Response

...

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2021
• Langue: anglais
• ISBN 10: 1071609025
• ISBN 13: 9781071609026
• Reliure: Broché
• Numéro d'édition: 2
• Nombre de pages: 292
• Éditeur: Maier Helena J., Bickerton Erica
• Coordonnées du fabricant: non disponible
• Personne responsable: CMN Printpool Inh. Mathis Neumann; https://www.also.com/ec/cms5/en_6000/6000/index.jsp; ; Friedrich-Karl-Str. 9; Hamburg; Allemagne