Periodontal Pathogens: Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1071609416 ISBN 13 : 9781071609415

Editeur : Humana, 2021

Synopsis

Part I: Methods for Bacterial Genetic Manipulation

1. Site-Directed and Random Mutagenesis in Porphyromonas gingivalis: Construction of Fimbriae-Related-Gene Mutant

So-ichiro Nishiyama, Yoshiaki Hasegawa, and Keiji Nagano

2. Genetic Manipulations of Oral Spirochete Treponema denticola

Kurni Kurniyati and Chunhao Li

3. Construction of a Gene-Deletion Mutant in Tannerella forsythia

Keiji Nagano and Yoshiaki Hasegawa

4. Construction of a Mutant in Prevotella melaninogenica Using the Conjugation Transfer Method with Escherichia coli

Yoshio Kondo

5. Genetic Transformation of Fusobacterium nucleatum

Akihiro Yoshida and Akihiko Ikegami

Part II: Experimental Methods to Examine Virulence Factors

6. Genotyping of Porphyromonas gingivalis in Relationship to Virulence

Atsuo Amano, Youn-Hee Choi, and Hiroki Takeuchi

7. Transport and Polymerization of Porphyromonas gingivalis Type V Pili

Mikio Shoji, Satoshi Shibata, Mariko Naito, and Koji Nakayama

8. Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis

Yoshiaki Hasegawa, Keiji Nagano, Yukitaka Murakami, and Richard J. Lamont

9. Crystallization of Recombinant Fimbrial Proteins of Porphyromonas gingivalis

Thomas Heidler and Karina Persson

10. Enzymatic Characteristics and Activities of Gingipains from Porphyromonas gingivalis

Tomoko Kadowaki

11. Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis

Dhana G. Gorasia, Eric Hanssen, Paul D. Veith, and Eric C. Reynolds

12. Methods for Functional Characterization of the Type IX Secretion System of Porphyromonas gingivalis

Keiko Sato

13. Purification of Tannerella forsythia Surface (S-) Layer Proteins

Sreedevi Chinthamani, Prasad R. Settem, Kiyonobu Honma, Takuma Nakajima, and Ashu Sharma

14. Separation of Glycosylated OmpA-Like Proteins from Porphyromonas gingivalis and Tannerella forsythia

Yukitaka Murakami, Keiji Nagano, and Yoshiaki Hasegawa

15. Intranasal Vaccine Study Using Porphyromonas gingivalis Membrane Vesicles: Isolation Method and Application to a Mouse Model

Satoru Hirayama and Ryoma Nakao

16. Analysis of the Butyrate-Producing Pathway in Porphyromonas gingivalis

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Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2021
• Langue: anglais
• ISBN 10: 1071609416
• ISBN 13: 9781071609415
• Reliure: Broché
• Nombre de pages: 268
• Éditeur: Nagano Keiji, Hasegawa Yoshiaki
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne