1. Overview of Human Cytomegalovirus Pathogenesis
Heather L. Fulkerson, Maciej T. Nogalski, Donna Collins-McMillen, and Andrew D. Yurochko
2. Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies
Giada Frascaroli and Christian Sinzger
3. Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection
Elizabeth A. Fortunato
4. The Use of Primary Human Cells to Analyze Human Cytomegalovirus Biology
Emma Poole, Ian Groves, Sarah Jackson, Mark Wills, and John Sinclair
5. Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation
Megan Peppenelli, Jason Buehler, and Felicia Goodrum
6. Collection and Isolation of CD14+ Primary Human Monocytes via Dual Density Centrifugation as a Model System to Study Human Cytomegalovirus Infection and Pathogenesis
Bailey S. Mosher, Heather Fulkerson, and Andrew D. Yurochko
7. Stable and Inducible Gene Knockdown in Primary Human Fibroblasts: A Versatile Tool to Study the Role of Human Cytomegalovirus Host Cell Factors
Anne-Charlotte Stilp, Patrick König, Myriam Scherer, and Thomas Stamminger
8. Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis
M. Zeeshan Chaudhry, Martin Messerle, and Luka Cicin-Sain
9. Methods for Studying the Function of Cytomegalovirus GPCRs
Christine M. O'Connor and William E. Miller
10. Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions
Jamil Mahmud and Gary C. Chan
11. Antibody-Independent Quantification of Cytomegalovirus Virion Protein Incorporation Using HiBiT
Iris K.A. Jones and Daniel N. Streblow
12. Use of a Phosphoproteomic Screen to Profile Early Changes during HCMV Infection of Human Monocytes
Liudmila S. Chesnokova and Andrew D. Yurochko
13. A Generally Applicable CRISPR/Cas9 Screening Technique to Identify Host Genes Required for Virus Infection as Applied to Human Cytomegalovirus (HCMV) Infection of Epithelial Cells
Xiaofei E and Timothy F. Kowalik
14. Quantitative Electron Microscopy to Study HCMV Morphogenesis
Clarissa Read, Paul Walther, and Jens von Einem
15. Detection of Cytomegalovirus Interleukin 10 (cmvIL-10) by Enzyme-Linked Immunosorbent Assay (ELISA)
Vivian P. Young, Margarette C. Mariano, Lionel Faure, and Juliet V. Spencer
16. Techniques for Characterizing Cytomegalovirus-Encoded miRNAs
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Gebunden. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsThis new edition explores and provides an update on the. N° de réf. du vendeur 389499858
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This new edition explores and provides an update on the biology and pathogenesis of human cytomegalovirus infection. Modern techniques that are currently being utilized to investigate the molecular aspects of viral infection, as well as how these new research studies are leading to new approaches to mitigate disease, are also provided. Given the key role the virus plays in significant acute and chronic human disease in all stages of life, from newborns to seniors, the need for clear methodologies to further explore the biology of HCMV infection and mitigation strategies is readily apparent. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second Edition serves as an ideal scientific reference for basic and clinical scientists and medical personnel on the modern understanding of the pathobiology of the virus, and the approaches, techniques, and models to study human cytomegalovirus infection and disease. 480 pp. Englisch. N° de réf. du vendeur 9781071611104
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Buch. Etat : Neu. Human Cytomegaloviruses | Methods and Protocols | Andrew D. Yurochko | Buch | xi | Englisch | 2021 | Springer US | EAN 9781071611104 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. N° de réf. du vendeur 118744712
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Buch. Etat : Neu. Neuware -This new edition explores and provides an update on the biology and pathogenesis of human cytomegalovirus infection. Modern techniques that are currently being utilized to investigate the molecular aspects of viral infection, as well as how these new research studies are leading to new approaches to mitigate disease, are also provided. Given the key role the virus plays in significant acute and chronic human disease in all stages of life, from newborns to seniors, the need for clear methodologies to further explore the biology of HCMV infection and mitigation strategies is readily apparent. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 480 pp. Englisch. N° de réf. du vendeur 9781071611104
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This new edition explores and provides an update on the biology and pathogenesis of human cytomegalovirus infection. Modern techniques that are currently being utilized to investigate the molecular aspects of viral infection, as well as how these new research studies are leading to new approaches to mitigate disease, are also provided. Given the key role the virus plays in significant acute and chronic human disease in all stages of life, from newborns to seniors, the need for clear methodologies to further explore the biology of HCMV infection and mitigation strategies is readily apparent. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second Edition serves as an ideal scientific reference for basic and clinical scientists and medical personnel on the modern understanding of the pathobiology of the virus, and the approaches, techniques, and models to study human cytomegalovirus infection and disease. N° de réf. du vendeur 9781071611104
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