Next Generation Culture Platforms for Reliable in Vitro Models: Methods and Protocols - Couverture rigide

9781071612453: Next Generation Culture Platforms for Reliable in Vitro Models: Methods and Protocols

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ISBN 10 : 107161245X ISBN 13 : 9781071612453

Editeur : Humana, 2021

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1. Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State

Alberto Maria Luciano, Rodrigo Garcia Barros, Ana Caroline Silva Soares, Jose Buratini, Valentina Lodde, and Federica Franciosi

2. Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids

Krzysztof Wrzesinski, Helle Sedighi Frandsen, Carlemi Calitz, Chrisna Gouws, Barbara Korzeniowska, and Stephen J. Fey

3. Protocol to Study the Role of Extracellular Vesicles during Induced Stem Cell Differentiation

Kelly C.S. Roballo, Carlos E. Ambrosio, and Juliano C. da Silveira

4. A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors

Malgorzata Duda, Lucia Gizler, and Gabriela Gorczyca

5. Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres

Jeffrey Aalders, Laurens L�ger, Davide Piras, Jolanda van Hengel, and Sergio Ledda

6. Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models

Antonio Murillo and Marta Mu�oz

7. Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues

Matteo Ghiringhelli, Yousef Abboud, Snizhanna V. Chorna, Irit Huber, Gil Arbel, Amira Gepstein, Georgia Pennarossa, Tiziana A.L. Brevini, and Lior Gepstein

8. Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models

Georgia Pennarossa, Alireza Fazeli, Sergio Ledda, Fulvio Gandolfi, and Tiziana A.L. Brevini

9. Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bio-Scaffold

Georgia Pennarossa, Matteo Ghiringhelli, Fulvio Gandolfi, and Tiziana A.L. Brevini

10. A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-Like Cells

Sharon Arcuri, Fulvio Gandolfi, Edgardo Somigliana, and Tiziana A.L. Brevini

11. 3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids

Joel Mario Vej-Nielsen and Adelina Rogowska-Wrzesinska

12. Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometry, Comet Assay, Transcriptomics, Proteomics, and Lipidomics

Helle Sedighi Frandsen, Martina Stampar, Joel Mario Vej-Nielsen, Bojana Zegura, and Adelina Rogowska-Wrzesinska

13. Isolation of Extracellular Vesicles (EVs) Using Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC)

Keerthie Dissan

Les informations fournies dans la section � Synopsis � peuvent faire r�f�rence � une autre �dition de ce titre.

�diteur: Humana
Date d'�dition: 2021
Langue: anglais
ISBN 10: 107161245X
ISBN 13: 9781071612453
Reliure: Reli�
Nombre de pages: 319
�diteur: Brevini Tiziana A.L., Fazeli Alireza, Turksen Kursad
Coordonn�es du fabricant: Springer Nature Customer Service Center GmbH
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Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols (eng)

Brevini, Tiziana A.L.

Edit� par Humana, 2021

ISBN 10 : 107161245X ISBN 13 : 9781071612453

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Next Generation Culture Platforms for Reliable in Vitro Models: Methods and Protocols

Brevini, Tiziana A.L.|Fazeli, Alireza|Turksen, Kursad

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step details essential for reproducible resultsContains key implementation advice from the expertsThis detailed book collects original protocols aimed a. N� de r�f. du vendeur 408051555

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Next Generation Culture Platforms for Reliable In Vitro Models

Tiziana A. L. Brevini

Edit� par Springer US Feb 2021, 2021

ISBN 10 : 107161245X ISBN 13 : 9781071612453

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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur in vivo. The protocols collected describe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other secreted vesicles, as well as micro and non-coding RNAs, that have been demonstrated to control the tuning of the in vivo micro and macro environment in order to ensure the optimal soluble environment in vitro. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols serves as an ideal guide for researchers working toward developing these vital testing models for preclinical studies. 320 pp. Englisch. N� de r�f. du vendeur 9781071612453

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Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols (Methods in Molecular Biology, 2273)

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Next Generation Culture Platforms for Reliable In Vitro Models : Methods and Protocols

Tiziana A. L. Brevini

Edit� par Springer, 2021

ISBN 10 : 107161245X ISBN 13 : 9781071612453

Neuf Couverture rigide

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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur in vivo. The protocols collected describe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other secreted vesicles, as well as micro and non-coding RNAs, that have been demonstrated to control the tuning of the in vivo micro and macro environment in order to ensure the optimal soluble environment in vitro. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols serves as an ideal guide for researchers working toward developing these vital testing models for preclinical studies. N� de r�f. du vendeur 9781071612453

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Next Generation Culture Platforms For Re

Brevini, Tiziana A.l

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Next Generation Culture Platforms for Reliable In Vitro Models

Tiziana A. L. Brevini

Edit� par Springer Feb 2021, 2021

ISBN 10 : 107161245X ISBN 13 : 9781071612453

Neuf Couverture rigide

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Buch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State.- Clinostat 3D Cell Culture: Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids.- Protocol to Study the Role of Extracellular Vesicles during Induced Stem Cell Differentiation.- A Simplified Method for Three-Dimensional (3D) Porcine Preantral Follicles Culture Utilizing Hydrophobic Microbioreactors.- Use of Transparent Liquid Marble: Microbioreactor to Culture Cardiospheres.- Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models.- Using Decellularization/Recellularization Processes to Prepare Liver and Cardiac Engineered Tissues.- Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models.- Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bio-Scaffold.- A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-Like Cells.- 3D-ViaFlow: A Quantitative Viability Assay for Multicellular Spheroids.- Method to Disassemble Spheroids into Core and Rim for Downstream Applications Such as Flow Cytometry, Comet Assay, Transcriptomics, Proteomics, and Lipidomics.- Isolation of Extracellular Vesicles (EVs) Using Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC).- Isolation of Extracellular Vesicles (EVs) Using Benchtop Size Exclusion Chromatography (SEC) Columns.- Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanoparticle Tracking Analyzer.- Isolation, Characterization, and MicroRNAs Analysis of Extracellular Vesicles from Bovine Oviduct and Uterine Fluids.- Biomimetic 3D-Bone Tissue Model.- Using the Air-Liquid Interface Approach to Foster Apical-Basal Polarization of Mammalian FemaleReproductive Tract Epithelia In Vitro.- Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Model the Fish Intestinal Mucosa.- Use of Porous Polystyrene Scaffolds to Bioengineer Human Epithelial Tissues In Vitro.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 320 pp. Englisch. N� de r�f. du vendeur 9781071612453

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