Flavin and Flavoproteins: Methods and Protocols - Couverture rigide

Name: Flavin and Flavoproteins: Methods and Protocols
ISBN: 9781071612859

9781071612859: Flavin and Flavoproteins: Methods and Protocols

Couverture rigide

ISBN 10 : 1071612859 ISBN 13 : 9781071612859

Editeur : Springer-Verlag New York Inc., 2021

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Part I: Riboflavin Production and Export by Microorganisms

1. Selection of Riboflavin Overproducing Strains of Lactic Acid Bacteria and Riboflavin Direct Quantification by Fluorescence

Pasquale Russo, Nicola De Simone, Vittorio Capozzi, Mari Luz Mohedano, Jos� �ngel Ruiz-Mas�, Gloria Del Solar, Paloma L�pez, and Giuseppe Spano

2. Recent Advances in Construction of the Efficient Producers of Riboflavin and Flavin Nucleotides (FMN, FAD) in the Yeast Candida Famata

Dariya V. Fedorovych, Kostyantyn V. Dmytruk, and Andriy A. Sibirny

3. Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida Famata

Andriy O. Tsyrulnyk, Dariya V. Fedorovych, Kostyantyn V. Dmytruk, and Andriy A. Sibirny

Part II: Measuring Riboflavin Transport and Flavin Cofactor Metabolism with Recombinant Human Proteins

4. Functional Study of the Human Riboflavin Transporter 2 Using Proteoliposomes System

Lara Console, Maria Tolomeo, and Cesare Indiveri

5. Heterologous Over-Expression of Human FAD Synthase Isoforms 1 and 2

Michele Galluccio and Cesare Indiveri

6. Purification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD / Protein Monomer Ratio by UV/Vis Spectra

Piero Leone, Stefano Quarta, Maria Tolomeo, and Maria Barile

7. Continuous and Discontinuous Approaches to Study FAD Synthesis and Degradation Catalyzed by Purified Recombinant FAD Synthase or Cellular Fractions

Piero Leone, Maria Tolomeo, and Maria Barile

Part III: Looking at Flavin Cofactors in Flavoenzymes

8. Redox Titration of Flavoproteins: An Overview

Francesco Bonomi and Stefania Iametti

9. Anaerobic Stopped-Flow Spectrophotometry with Photodiode Array Detection in the Pre-Steady State: An Application to Elucidate Oxido-Reduction Mechanisms in Flavoproteins

Patricia Ferreira and Milagros Medina

10. Atomic Force Microscopy: Single Molecule Imaging and Force Spectroscopy in the Study of Flavoproteins Ligand Binding and Reaction Mechanisms

Anabel Lostao and Milagros Medina

11. Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD⁺ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State

Paolo Cocomazzi, Luca Sorrentino, Federica Cossu, and Alessandro Aliverti

12. Ligand Binding in Allosteric Flavoproteins: Part 2. Quantitative Analysis of the Redox-Dependent Interaction of the Apoptosis-Inducing Factor (AIF) with Its Protein Partner

Paolo Cocomazzi, Delia Tarantino, Eloise Mastrangelo, and Alessandro Aliverti

13. Using D- and L-Amino Acid Oxidases to Generate the Imino Acid Substrate to Measure the Activity

Les informations fournies dans la section � Synopsis � peuvent faire r�f�rence � une autre �dition de ce titre.

�diteur: Springer-Verlag New York Inc.
Date d'�dition: 2021
Langue: anglais
ISBN 10: 1071612859
ISBN 13: 9781071612859
Reliure: Reli�
Nombre de pages: 311
�diteur: Barile Maria
Coordonn�es du fabricant: Springer Nature Customer Service Center GmbH
ProductSafety@springernature.com

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Flavins and Flavoproteins (eng)

Barile, Maria

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Flavin and Flavoproteins: Methods and Protocols

Barile, Maria

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Gebunden. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsIncludes cutting-edge . N� de r�f. du vendeur 416589808

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Flavins and Flavoproteins

Maria Barile

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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells. Starting with Rf production in microorganisms and the chemical, optical, and redox properties of these fascinating molecules and moving along to the variety and the peculiarity of some single flavoenzymes, the volume explores the complexity of functions and distribution of these molecules in the cell. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Flavin and Flavoproteins: Methods and Protocols serves as an ideal guide for protein chemists interested in purifying and characterizing flavoproteins, as well as microbiologists, physiologists, and clinicians, who wish to further study problems connected with flavoproteins. 312 pp. Englisch. N� de r�f. du vendeur 9781071612859

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Flavins and Flavoproteins: Methods and Protocols (Methods in Molecular Biology, 2280)

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Flavins and Flavoproteins : Methods and Protocols

Maria Barile

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ISBN 10 : 1071612859 ISBN 13 : 9781071612859

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Buch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells. Starting with Rf production in microorganisms and the chemical, optical, and redox properties of these fascinating molecules and moving along to the variety and the peculiarity of some single flavoenzymes, the volume explores the complexity of functions and distribution of these molecules in the cell. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Flavin and Flavoproteins: Methods and Protocols serves as an ideal guide for protein chemists interested in purifying and characterizing flavoproteins, as well as microbiologists, physiologists, and clinicians, who wish to further study problems connected with flavoproteins. N� de r�f. du vendeur 9781071612859

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Flavins and Flavoproteins

Maria Barile

Edit� par Springer, Humana M�r 2021, 2021

ISBN 10 : 1071612859 ISBN 13 : 9781071612859

Neuf Couverture rigide

impression � la demande

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Buch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells. Starting with Rf production in microorganisms and the chemical, optical, and redox properties of these fascinating molecules and moving along to the variety and the peculiarity of some single flavoenzymes, the volume explores the complexity of functions and distribution of these molecules in the cell. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 312 pp. Englisch. N� de r�f. du vendeur 9781071612859

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