Articles liés à Flavins and Flavoproteins: Methods and Protocols
Synopsis
Part I: Riboflavin Production and Export by Microorganisms
1. Selection of Riboflavin Overproducing Strains of Lactic Acid Bacteria and Riboflavin Direct Quantification by Fluorescence
Pasquale Russo, Nicola De Simone, Vittorio Capozzi, Mari Luz Mohedano, José Ángel Ruiz-Masó, Gloria Del Solar, Paloma López, and Giuseppe Spano
2. Recent Advances in Construction of the Efficient Producers of Riboflavin and Flavin Nucleotides (FMN, FAD) in the Yeast Candida Famata
Dariya V. Fedorovych, Kostyantyn V. Dmytruk, and Andriy A. Sibirny
3. Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida Famata
Andriy O. Tsyrulnyk, Dariya V. Fedorovych, Kostyantyn V. Dmytruk, and Andriy A. Sibirny
Part II: Measuring Riboflavin Transport and Flavin Cofactor Metabolism with Recombinant Human Proteins
4. Functional Study of the Human Riboflavin Transporter 2 Using Proteoliposomes System
Lara Console, Maria Tolomeo, and Cesare Indiveri
5. Heterologous Over-Expression of Human FAD Synthase Isoforms 1 and 2
Michele Galluccio and Cesare Indiveri
6. Purification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD / Protein Monomer Ratio by UV/Vis Spectra
Piero Leone, Stefano Quarta, Maria Tolomeo, and Maria Barile
7. Continuous and Discontinuous Approaches to Study FAD Synthesis and Degradation Catalyzed by Purified Recombinant FAD Synthase or Cellular Fractions
Piero Leone, Maria Tolomeo, and Maria Barile
Part III: Looking at Flavin Cofactors in Flavoenzymes
8. Redox Titration of Flavoproteins: An Overview
Francesco Bonomi and Stefania Iametti
9. Anaerobic Stopped-Flow Spectrophotometry with Photodiode Array Detection in the Pre-Steady State: An Application to Elucidate Oxido-Reduction Mechanisms in Flavoproteins
Patricia Ferreira and Milagros Medina
10. Atomic Force Microscopy: Single Molecule Imaging and Force Spectroscopy in the Study of Flavoproteins Ligand Binding and Reaction Mechanisms
Anabel Lostao and Milagros Medina
11. Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State
Paolo Cocomazzi, Luca Sorrentino, Federica Cossu, and Alessandro Aliverti
12. Ligand Binding in Allosteric Flavoproteins: Part 2. Quantitative Analysis of the Redox-Dependent Interaction of the Apoptosis-Inducing Factor (AIF) with Its Protein Partner
Paolo Cocomazzi, Delia Tarantino, Eloise Mastrangelo, and Alessandro Aliverti
13. Using D- and L-Amino Acid Oxidases to Generate the Imino Acid Substrate to Measure the Activity
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Paperback. Etat : new. Paperback. This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells. Starting with Rf production in microorganisms and the chemical, optical, and redox properties of these fascinating molecules and moving along to the variety and the peculiarity of some single flavoenzymes, the volume explores the complexity of functions and distribution of these molecules in the cell. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Flavin and Flavoproteins: Methods and Protocols serves as an ideal guide for protein chemists interested in purifying and characterizing flavoproteins, as well as microbiologists, physiologists, and clinicians, who wish to further study problems connected with flavoproteins. This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. N° de réf. du vendeur 9781071612880
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ISBN 13 : 9781071612880
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Taschenbuch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells. Starting with Rf production in microorganisms and the chemical, optical, and redox properties of these fascinating molecules and moving along to the variety and the peculiarity of some single flavoenzymes, the volume explores the complexity of functions and distribution of these molecules in the cell. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Flavin and Flavoproteins: Methods and Protocols serves as an ideal guide for protein chemists interested in purifying and characterizing flavoproteins, as well as microbiologists, physiologists, and clinicians, who wish to further study problems connected with flavoproteins. N° de réf. du vendeur 9781071612880
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Flavins and Flavoproteins (Paperback)
Edité par
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ISBN 10 : 1071612883
ISBN 13 : 9781071612880
Neuf
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Paperback. Etat : new. Paperback. This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells. Starting with Rf production in microorganisms and the chemical, optical, and redox properties of these fascinating molecules and moving along to the variety and the peculiarity of some single flavoenzymes, the volume explores the complexity of functions and distribution of these molecules in the cell. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Flavin and Flavoproteins: Methods and Protocols serves as an ideal guide for protein chemists interested in purifying and characterizing flavoproteins, as well as microbiologists, physiologists, and clinicians, who wish to further study problems connected with flavoproteins. This book of protocols is devoted to the yellow coenzymes derived from riboflavin or vitamin B2 and to the hundreds of enzymes whose functionality depends on them, and represents a compendium of techniques for working with flavoproteins or with the wide spectrum of functions that flavoproteins can drive in the cells. Shipping may be from our Sydney, NSW warehouse or from our UK or US warehouse, depending on stock availability. N° de réf. du vendeur 9781071612880
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