Articles liés à Myofibroblasts: Methods and Protocols
Synopsis
1. 50 Years of Myofibroblasts: How the Myofibroblast Concept Evolved
Giulio Gabbiani
Section I: Fundamental Methods to Study Myofibroblast Biology
2. Myofibroblast Functions in Tissue Repair and Fibrosis: An Introduction
Victor J. Thannickal
3. Myofibroblast Markers and Microscopy Detection Methods in Cell Culture and Histology
Fereshteh Younesi, Dong Ok Son, Joao Firmino, and Boris Hinz
4. Fibroblast and Myofibroblast Subtypes: Single Cell Sequencing
Hesham Soliman, Lin Wei Tung, and Fabio M.V. Rossi
5. Myofibroblast Adhesome Analysis by Mass Spectrometry
Christopher A. McCulloch
6. Myofibroblast TGF-β Activation Measurement In Vitro
Joanne Porte, Gisli Jenkins, and Amanda L. Tatler
7. Contraction Measurements Using Three-Dimensional Fibrillar Collagen Gel Lattices
James Griffith and William L. Berry
8. Techniques to Assess Collagen Synthesis, Deposition, and Cross-Linking In Vitro
Tamara Rosell-García and Fernando Rodriguez-Pascual
9. Methods for Studying Myofibroblast Apoptotic Pathways
Yan Zhou and David Lagares
10. Determination of Senescent Myofibroblasts in Precision-Cut Lung Slices
Tamara Cruz, Ana L. Mora, and Mauricio Rojas
11. The Scar-in-a-Jar: In Vitro Fibrosis Model for Anti-Fibrotic Drug Testing
Simon Stebler and Michael Raghunath
Section II: Myofibroblast Mechanobiology
12. Why Stress Matters: An Introduction
Daniel J. Tschumperlin
13. Soft Substrate Culture to Mechanically Control Cardiac Myofibroblast Activation
Natalie M. Landry, Sunil G. Rattan, and Ian M.C. Dixon
14. Quantitative Analysis of Myofibroblast Contraction by Traction Force Microscopy
Shuying Yang, Fernando R. Valencia, Benedikt Sabass, and Sergey V. Plotnikov
15. Nucleocytoplasmic Shuttling of the Mechanosensitive Transcription Factors MRTF and YAP/TAZ
Michael Kofler and András Kapus
16. Atomic Force Microscopy for Live-Cell and Hydrogel Measurement
Alexander Whitehead, Natalie J. Kirkland, and Adam J. Engler
17. Method for Investigating Fibroblast Durotaxis
Hossam Kadry, David Lagares, and Taslim A. Al-Hilal
18. Decellularized Extracellular Matrix (ECM) as a Model to Study Fibrotic ECM Mecha
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Myofibroblasts
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsIncludes cutting-edge techniquesProvides step. N° de réf. du vendeur 426650169
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Myofibroblasts: Methods and Protocols (Methods in Molecular Biology, 2299)
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Myofibroblasts
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Springer US Mai 2021, 2021
ISBN 10 : 1071613812
ISBN 13 : 9781071613818
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book explores the fundamentals of myofibroblast biology in tissue repair, fibrosis, and tumors as well as providing cutting-edge laboratory methods used to investigate myofibroblast functions in physiological and pathological settings in vitro and in vivo, as written by leading academic scientists. Section I of this volume focuses on fundamental methods to study myofibroblast biology and covers topics such as methods for detecting myofibroblasts and senescent myofibroblasts in cell culture and histology, single cell RNA sequencing to identify myofibroblast subsets in fibrotic tissues, and functional assays to assess TGF-beta activation, myofibroblast apoptosis, or matrix deposition and crosslinking. Section II discusses methods to investigate the mechanobiology of myofibroblasts in vitro, including the fabrication of functional hydrogels with tunable stiffness, the use of atomic force microscopy to characterize matrix and cellular stiffness, as well as molecular assays to assess fibroblast mechanotransduction pathways and durotaxis. Section III describes multiple animal models to investigate myofibroblast functions across organs in vivo as well as human organoid systems, precision tissue slices and decellularized 3D tissue scaffolds to assess myofibroblast functions in relevant human ex vivo models. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and extensive,Myofibroblasts: Methods and Protocolsis an essential collection for researchers delving into the processes and effects of these important cells. 476 pp. Englisch. N° de réf. du vendeur 9781071613818
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Myofibroblasts
Edité par
Springer US, Springer US Mai 2021, 2021
ISBN 10 : 1071613812
ISBN 13 : 9781071613818
Neuf
Couverture rigide
Vendeur : buchversandmimpf2000, Emtmannsberg, BAYE, Allemagne
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Buch. Etat : Neu. Neuware -This detailed book explores the fundamentals of myofibroblast biology in tissue repair, fibrosis, and tumors as well as providing cutting-edge laboratory methods used to investigate myofibroblast functions in physiological and pathological settings in vitro and in vivo, as written by leading academic scientists. Section I of this volume focuses on fundamental methods to study myofibroblast biology and covers topics such as methods for detecting myofibroblasts and senescent myofibroblasts in cell culture and histology, single cell RNA sequencing to identify myofibroblast subsets in fibrotic tissues, and functional assays to assess TGF-¿ activation, myofibroblast apoptosis, or matrix deposition and crosslinking. Section II discusses methods to investigate the mechanobiology of myofibroblasts in vitro, including the fabrication of functional hydrogels with tunable stiffness, the use of atomic force microscopy to characterize matrix and cellular stiffness, as well as molecular assays to assess fibroblast mechanotransduction pathways and durotaxis. Section III describes multiple animal models to investigate myofibroblast functions across organs in vivo as well as human organoid systems, precision tissue slices and decellularized 3D tissue scaffolds to assess myofibroblast functions in relevant human ex vivo models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 476 pp. Englisch. N° de réf. du vendeur 9781071613818
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Myofibroblasts : Methods and Protocols
Edité par
Springer US, Springer US, 2021
ISBN 10 : 1071613812
ISBN 13 : 9781071613818
Neuf
Couverture rigide
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed book explores the fundamentals of myofibroblast biology in tissue repair, fibrosis, and tumors as well as providing cutting-edge laboratory methods used to investigate myofibroblast functions in physiological and pathological settings in vitro and in vivo, as written by leading academic scientists. Section I of this volume focuses on fundamental methods to study myofibroblast biology and covers topics such as methods for detecting myofibroblasts and senescent myofibroblasts in cell culture and histology, single cell RNA sequencing to identify myofibroblast subsets in fibrotic tissues, and functional assays to assess TGF-beta activation, myofibroblast apoptosis, or matrix deposition and crosslinking. Section II discusses methods to investigate the mechanobiology of myofibroblasts in vitro, including the fabrication of functional hydrogels with tunable stiffness, the use of atomic force microscopy to characterize matrix and cellular stiffness, as well as molecular assays to assess fibroblast mechanotransduction pathways and durotaxis. Section III describes multiple animal models to investigate myofibroblast functions across organs in vivo as well as human organoid systems, precision tissue slices and decellularized 3D tissue scaffolds to assess myofibroblast functions in relevant human ex vivo models. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and extensive,Myofibroblasts: Methods and Protocolsis an essential collection for researchers delving into the processes and effects of these important cells. N° de réf. du vendeur 9781071613818
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Myofibroblasts: Methods and Protocols (Methods in Molecular Biology, 2299)
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Myofibroblasts: Methods and Protocols (Methods in Molecular Biology, 2299)
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Myofibroblasts: Methods and Protocols (Methods in Molecular Biology, 2299)
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Myofibroblasts: Methods and Protocols
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Hardcover. Etat : Brand New. 475 pages. 10.00x7.00x1.22 inches. In Stock. N° de réf. du vendeur x-1071613812
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