Cytochrome P450: In Vitro Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1071615440 ISBN 13 : 9781071615447

Editeur : Humana, 2022

Synopsis

This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading.
Authoritative and practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2022
• Langue: anglais
• ISBN 10: 1071615440
• ISBN 13: 9781071615447
• Reliure: Broché
• Nombre de pages: 392
• Éditeur: Yan Zhengyin, Caldwell Gary W.
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne