1. Inference of Ligand-Receptor Pairs from Single-Cell Transcriptomics Data
Mirjana Efremova and Roser Vento-Tormo
2. Multiple Imaging Modalities for Cell-Cell Communication via Calcium Mobilizations in Corneal Epithelial Cells
Yoonjoo K. Lee, Kristen L. Segars, and Vickery Trinkaus-Randall
3. Interactions of Hematopoietic Stem Cells with Bone Marrow Niche
Xinghui Zhao, Cuiping Zhang, Xiaojing Cui, and Ying Liang
4. Ex Vivo Modeling of Hematopoietic Stem Cell Homing to the Fetal Liver
Amina Mohammadalipour, Miguel F. Diaz, Sumedha Pareek, and Pamela L. Wenzel
5. Analysis of Epithelial Architecture and Planar Spindle Orientation in the Drosophila Wing Disc
Yu-ichiro Nakajima
6. A Co-Culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival
Ling Lin, Samantha White, and Kebin Hu
7. Calcium Fluorescence Recordings from Neuroepithelial Stem Cells
Masayuki Yamashita
8. Ultrastructural Analysis of Cell-Cell Interactions in Drosophila Ovary
Matthew Antel, Valentina Baena, Mark Terasaki, and Mayu Inaba
9. TIRF Microscopy as a Tool to Determine Exosome Composition
Noa B. Martín-Cófreces, Daniel Torralba, Marta Lozano-Prieto, Nieves Fernández-Gallego, and Francisco Sánchez-Madrid
10. Rapid Visualization of Intracellular Vesicle Events during Synaptic Stimulation
Noa B. Martín-Cófreces, Amelia Rojas-Gomez, Sara G. Dosil, Irene Fernandez-Delgado, and Francisco Sánchez-Madrid
11. Monitoring of Active Notch Signaling in Mouse Bladder Urothelium
Panagiotis Karakaidos and Theodoros Rampias
12. Examining Local Cell-to-Cell Signaling in the Kidney Using ATP Biosensing
Gareth W. Price, Joe A. Potter, Bethany M. Williams, Chelsy L. Cliff, Mark J. Wall, Claire E. Hills, and Paul E. Squires
13. Isolation and Assessment of Pancreatic Islets Versus Dispersed Beta Cells: A Straightforward Approach to Examine Cell-Cell Communication
Rachel T. Scarl, William J. Koch, Kathryn L. Corbin, and Craig S. Nunemaker
14. Promoter Pull-Down Assay: A Biochemical Screen for DNA-Binding Proteins
Ryan R. Chaparian and Julia C. van Kessel
15. Purification of the Vibrio Quorum-Sensing Transcription Factors LuxR, HapR, and SmcR
Jane D. Newman and Julia C. van Kessel
16. Preserving Cytonemes for Immunocytochemistry of Cultured Adherent Cells
Sally Rogers and Steffen Scholpp
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book brings together a new set of protocols to arm cell biologists with techniques that are currently being used in a number of well-established laboratories around the world. The contents represent the great strides made in the field of cell-cell communications with respect to the identification and characterization of key components of the communication apparatus, assembly and maintenance of the communications structures, and concomitantly their roles in not only tissue formation and maintenance but also regeneration and repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols, Second Edition serves as an ideal guide for experts and newcomers alike seeking to increase our understanding of the crucial biological and physiological roles of cell-cell communications in tissue function and organismal integrity. 256 pp. Englisch. N° de réf. du vendeur 9781071615690
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