Part I: Introduction to Fibrous Proteins
1. The Structure of Animal Silks
Wenwen Zhang and Yimin Fan
2. The Structure of Collagen
Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren
3. The Structure of Elastin
Yuelong Xiao, Shengjie Ling, and Ying Pei
4. The Structure of Resilin
Yuelong Xiao, Shengjie Ling, and Ying Pei
5. The Structure of Keratin
Wenwen Zhang and Yimin Fan
Part II: Methods in Synthesis of Fibrous Proteins
6. General Methods to Produce and Assemble Recombinant Spider Silk Proteins
Na Kong
7. Self-Assembly of Bombyx mori Silk Fibroin
Na Kong
8. Synthesis and Assembly of Recombinant Collagen
Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren
9. Recombination and Purification of Elastin-Like Polypeptides
Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren
10. Methods to Synthesize and Assemble Recombinant Keratins
Wenwen Zhang and Yimin Fan
11. Preparation of Amyloid Fibers Using Recombinant Technology
Wenwen Zhang and Yimin Fan
12. Molecular Biology Methods to Construct Recombinant Fibrous Protein
Jicong Zhang
Part III: Methods in Assembly of Fibrous Proteins
13. Isolation of Nanofibrils from Animal Silks
Ke Zheng
14. Gelation Methods to Assemble Fibrous Proteins
Ning Fan and Ke Zheng
15. Spinning Methods Used for The Construction of One- and Two-Dimensional Fibrous Protein Materials
Leitao Cao
16. Three-Dimensional Printing to Build Fibrous Protein Architectures
Huanhuan Qiao and Ke Zheng
Part IV: Methods in Characterization of Fibrous Proteins
17. The Methods of Using Synchrotron FTIR Microspectroscopy to Understand the Conformation of Single Animal Silk Fibers
Chao Ye, Leitao Cao, and Shengjie Ling
18. The Protocol of Using FTIR Imaging to Investigate Silk Fibroin-Based Materials
Jiajia Zhong, Xiaojie Zhou, Chao Ye, Wenjie Yu, and Yuzhao Tang
19. Secondary Structure Analysis of Single Silk Nanofibril through Infrared Nanospectroscopy
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsIncludes cutting-edge techniquesProvides step. N° de réf. du vendeur 455019721
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores fibrous proteins widely present in different biological tissues or biological structural materials. The book begins by introducing the structure of representative fibrous proteins, including animal silks, collagen, elastin, resilin, and keratin, and it then continues by providing detailed experimental protocols for the synthesis, assembly, and characterization of natural, regenerated, and recombinant fibrous proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Fibrous Proteins: Design, Synthesis, and Assembly is an ideal guide for researchers aiming to master fibrous protein preparations with the aid of this broad and interdisciplinary perspective on understanding the structure-property-function relationships of natural and reconstituted fibrous proteins. 276 pp. Englisch. N° de réf. du vendeur 9781071615737
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