Enhancers and Promoters: Methods and Protocols - Couverture souple

Name: Enhancers and Promoters: Methods and Protocols
ISBN: 9781071615997

Livre 99 sur 381: Methods in Molecular Biology

9781071615997: Enhancers and Promoters: Methods and Protocols

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ISBN 10 : 1071615998 ISBN 13 : 9781071615997

Editeur : Humana, 2022

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This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays. Written in the Methods in Molecular Biology series format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting.

Authoritative and cutting-edge, Enhancers and Promoters: Methods and Protocols is a useful guide for future experiments.
Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com

Les informations fournies dans la section � Synopsis � peuvent faire r�f�rence � une autre �dition de ce titre.

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Les informations fournies dans la section � A propos du livre � peuvent faire r�f�rence � une autre �dition de ce titre.

�diteur: Humana
Date d'�dition: 2022
Langue: anglais
ISBN 10: 1071615998
ISBN 13: 9781071615997
Reliure: Broch�
Nombre de pages: 376
�diteur: Borggrefe Tilman, Giaimo Benedetto Daniele
Coordonn�es du fabricant: ProductSafety@springernature.com
ProductSafety@springernature.com

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Enhancers and Promoters (eng)

Borggrefe, Tilman

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Enhancers and Promoters

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the expertsThis volume contains cutting-edge techniques to study the func. N� de r�f. du vendeur 668435935

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Enhancers and Promoters: Methods and Protocols

Tilman Borggrefe

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ISBN 10 : 1071615998 ISBN 13 : 9781071615997

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Paperback / softback. Etat : New. This item is printed on demand. New copy - Usually dispatched within 5-9 working days. N� de r�f. du vendeur C9781071615997

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Enhancers and Promoters : Methods and Protocols

Benedetto Daniele Giaimo

Edit� par Humana, 2022

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Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - A New Toolbox to Analyze Enhancer-Promoter Functions.- Global Run-on Sequencing (GRO-Seq).- Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET-Seq).- Low Quantity single strand CAGE (LQ-ssCAGE) Maps Regulatory Enhancers and Promoters.- Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase-Seq.- Measuring Chromatin Accessibility: ATAC-Seq.- High-resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles.- Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes.- Low Input Targeted Chromatin Capture (low-T2C).- Proximity Ligation-assisted ChIP-Seq (PLAC-Seq).- Analysis of Enhancer-promoter Interactions using CAGE and RADICL-Seq technologies.- Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to identify open chromatin interactions.- Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C).- Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-Translational Modification (PTM) Combinatorics At Distinct Genomic Regions.- Using ChIP-SICAP to Identify Proteins That Co-Localize in Chromatin.- Genome-wide profiling of protein-DNA Interactions with Chromatin Endogenous Cleavage And High-Throughput Sequencing (ChEC-Seq).- Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators.- Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors.- Simultaneous Tagmentation-based detection of ChIP/ATAC Signal with Bisulfite Sequencing.- Low Input Whole-Genome Bisulfite Sequencing. N� de r�f. du vendeur 9781071615997

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Enhancers and Promoters

Tilman Borggrefe

Edit� par Humana Aug 2022, 2022

ISBN 10 : 1071615998 ISBN 13 : 9781071615997

Neuf Taschenbuch

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays. Written in the Methods in Molecular Biology series format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting.Authoritative and cutting-edge, Enhancers and Promoters: Methods and Protocols is a useful guide for future experiments.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 392 pp. Englisch. N� de r�f. du vendeur 9781071615997

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