1. Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts
Juan M. Adrian-Segarra, Bettina Weigel, and Moritz Mall
2. Direct In Vitro Reprogramming of Astrocytes into Induced Neurons
Nesrin Sharif, Filippo Calzolari, and Benedikt Berninger
3. Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9
Boning Qiu, Ruben J. de Vries, and Massimiliano Caiazzo
4. Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules
E Tian, Mingzi Zhang, and Yanhong Shi
5. Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons
Amel Falco, Rocío Bartolomé-Cabrero, and Sergio Gascón
6. Direct Conversion of Human Fibroblasts to Induced Neurons
Lucia Zhou-Yang, Sophie Eichhorner, Lukas Karbacher, Lena Böhnke, Larissa Traxler, and Jerome Mertens
7. Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts
Emilie M. Legault and Janelle Drouin-Ouellet
8. Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs)
Ruiqi Hu, Xiaoting Zhu, and Nan Yang
9. Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors
Keiko Imamura, Jitsutaro Kawaguchi, Tsugumine Shu, and Haruhisa Inoue
10. Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons
Ella Quist, Henrik Ahlenius, and Isaac Canals
11. Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression
Katrien Neyrinck and Juan A. García-León
12. Transcriptional Profiling During Neural Conversion
Yohannes Afeworki, Hannah Wollenzien, and Michael S. Kareta
13. Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo
Srisaiyini Kidnapillai and Daniella Rylander Ottosson
14. Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods
Andreas Heuer
15. Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions
Juan M. Adrian-Segarra, Bettina Weigel, and Moritz Mall
16. CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders
Isaac Canals and H
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as well as in silico methods to aid reprogramming efforts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Neural Reprogramming: Methods and Protocols provides ample experimental experience and guidance for anyone, be it experienced researcher or beginner, to generate, validate, and apply reprogrammed neural cells in their research. 280 pp. Englisch. N° de réf. du vendeur 9781071616000
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Buch. Etat : Neu. Neuware -This detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as well as in silico methods to aid reprogramming efforts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 280 pp. Englisch. N° de réf. du vendeur 9781071616000
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