Neural Reprogramming: Methods and Protocols - Couverture rigide

Couverture rigide

ISBN 10 : 1071616005 ISBN 13 : 9781071616000

Editeur : Springer-Verlag New York Inc., 2021

Synopsis

1. Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts

Juan M. Adrian-Segarra, Bettina Weigel, and Moritz Mall

2. Direct In Vitro Reprogramming of Astrocytes into Induced Neurons

Nesrin Sharif, Filippo Calzolari, and Benedikt Berninger

3. Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9

Boning Qiu, Ruben J. de Vries, and Massimiliano Caiazzo

4. Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules

E Tian, Mingzi Zhang, and Yanhong Shi

5. Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons

Amel Falco, Rocío Bartolomé-Cabrero, and Sergio Gascón

6. Direct Conversion of Human Fibroblasts to Induced Neurons

Lucia Zhou-Yang, Sophie Eichhorner, Lukas Karbacher, Lena Böhnke, Larissa Traxler, and Jerome Mertens

7. Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts

Emilie M. Legault and Janelle Drouin-Ouellet

8. Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs)

Ruiqi Hu, Xiaoting Zhu, and Nan Yang

9. Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors

Keiko Imamura, Jitsutaro Kawaguchi, Tsugumine Shu, and Haruhisa Inoue

10. Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons

Ella Quist, Henrik Ahlenius, and Isaac Canals

11. Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression

Katrien Neyrinck and Juan A. García-León

12. Transcriptional Profiling During Neural Conversion

Yohannes Afeworki, Hannah Wollenzien, and Michael S. Kareta

13. Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo

Srisaiyini Kidnapillai and Daniella Rylander Ottosson

14. Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods

Andreas Heuer

15. Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions

Juan M. Adrian-Segarra, Bettina Weigel, and Moritz Mall

16. CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders

Isaac Canals and H

Détails de l'édition

• Éditeur: Springer-Verlag New York Inc.
• Date d'édition: 2021
• Langue: anglais
• ISBN 10: 1071616005
• ISBN 13: 9781071616000
• Reliure: Relié
• Nombre de pages: 263
• Éditeur: Ahlenius Henrik
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Allemagne