Essential Genes and Genomes: Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1071617222 ISBN 13 : 9781071617229

Editeur : Humana, 2022

Synopsis

1. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9

Barbara Mair, Michael Aregger, Amy H.Y. Tong, Katherine S.K. Chan, and Jason Moffat

2. CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines

Kimberly S. Huggler, Nicholas J. Rossiter, Kyle M. Flickinger, and Jason R. Cantor

3. Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes

Bert Kwanten, Jasper Neggers, and Dirk Daelemans

4. Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen Candida albicans

Lauren Wensing and Rebecca S. Shapiro

5. Identification of Essential Genes Using Sequential CRISPR and siRNA Screens

Luke DeHart, Oliver P. Yockey, and Jesse Bakke

6. A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines

Kohei Nishimura and Tatsuo Fukagawa

7. New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference Screen

Xihao Liao, Xin-Hui Xing, and Chong Zhang

8. A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria

Ju-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, and Cheng-Cai Zhang

9. A Method of Transposon Insertion Sequencing in Comprehensively Identifying Vibrio vulnificus Genes Required for Growth in Human Serum

Miguel Carda-Diéguez and Carmen Amaro

10. The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of Pseudomonas aeruginosa

Bradley E. Poulsen, Anne E. Clatworthy, and Deborah T. Hung

11. Analysis of Escherichia coli K1 Virulence Genes by Transposon-Directed Sequencing

Alex J. McCarthy and Peter W. Taylor

12. Using Genome Scale Mutant Libraries to Identify Essential Genes

Kevin S. Myers, Piyush Lal, Daniel R. Noguera, and Timothy J. Donohue

13. Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing

Alejandro R. Walker and Robert C. Shields

14. The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle Systems

Gayatri Sharma and Patrick D. Curtis

15. A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2022
• Langue: anglais
• ISBN 10: 1071617222
• ISBN 13: 9781071617229
• Reliure: Broché
• Nombre de pages: 448
• Éditeur: Zhang Ren
• Coordonnées du fabricant: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Allemagne