Part I: Bioinformatics
1. Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression
Eliana Destefanis and Erik Dassi
2. Predicting RNA Secondary Structure Using In Vitro and In Vivo Data
Riccardo Delli Ponti and Gian Gaetano Tartaglia
3. RBPmap: A Tool for Mapping and Predicting the Binding Sites of RNA-Binding Proteins Considering the Motif Environment
Inbal Paz, Amir Argoetti, Noa Cohen, Niv Even, and Yael Mandel-Gutfreund
Part II: Expression Studies
4. Analysis of mRNA Translation by Polysome Profiling
Anne Cammas, Pauline Herviou, Leïla Dumas, and Stefania Millevoi
5. Exploring Ribosome-Positioning on Translating Transcripts with Ribosome Profiling
Alexander L. Cope, Sangeevan Vellappan, John S. Favate, Kyle S. Skalenko, Srujana S. Yadavalli, and Premal Shah
6. Identification of RNA Binding Partners of CRISPR-Cas Proteins in Prokaryotes Using RIP-Seq
Sahil Sharma and Cynthia M. Sharma
7. Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems
Katharina G. Wandera and Chase L. Beisel
Part III: Interactomics
8. Studying RNP Composition with RIP
Annalisa Rossi and Alberto Inga
9. PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites
Charles Danan, Sudhir Manickavel, and Markus Hafner
10. A Pipeline for Analyzing eCLIP and iCLIP Data with htseq-clip and DEWSeq
Sudeep Sahadevan, Thileepan Sekaran, and Thomas Schwarz
11. Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH)
Andrea Zeni, Margherita Grasso, and Michela A. Denti
12. Identifying the Protein Interactomes of Target RNAs Using HyPR-MS
Katherine B. Henke, Rachel M. Miller, Rachel A. Knoener, Mark Scalf, Michele Spiniello, and Lloyd M. Smith
Part IV: The RNA Lifecycle
13. Visualization and Quantification of Subcellular RNA Localization Using Single Molecule RNA Fluorescence In Situ Hybridization
Ankita Arora, Raeann Goering, Pedro Tirado Velez, and J. Matthew Taliaferro
14. Single-Molecule RNA Imaging Using Mango II Arrays
Adam D. Cawte, Haruki Iino, Peter J. Unrau, and David S. Rueda
15. Genome-Wide Identification of Polyadenylation Dynamics with TED-Seq
Yeonui Kwak and Hojoong Kwa
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Kartoniert / Broschiert. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsThis volume presents the most recent advances in techniques for studying the post-tran. N° de réf. du vendeur 727137401
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Taschenbuch. Etat : Neu. Post-Transcriptional Gene Regulation | Erik Dassi | Taschenbuch | xiv | Englisch | 2022 | Humana | EAN 9781071618530 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. N° de réf. du vendeur 125331085
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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume presents the most recent advances in techniques for studying the post-transcriptional regulation of gene expression (PTR). With sections on bioinformatics approaches, expression profiling, the protein and RNA interactome, the mRNA lifecycle, and RNA modifications, the book guides molecular biologists toward harnessing the power of this new generation of techniques, while also introducing the data analysis skills that these high-throughput techniques require. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 428 pp. Englisch. N° de réf. du vendeur 9781071618530
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