Synopsis
Part I: Virus and Virus Protein Production
1. Virus Propagation and Plaque Assay for Dengue Virus
Pedro Henrique Carneiro, Lucas Mendes-Monteiro, and Ronaldo Mohana-Borges
2. Transmission Electron Microscopy Studies of Dengue Viruses
Gabriela Cardoso Caldas, Ortrud Monika Barth, and Debora Ferreira Barreto-Vieira
3. Production of Properly Folded NS1 Protein in Bacterial Cells
Diego Allonso
4. Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System
Diego R. Coelho and Pedro Henrique Carneiro
5. Purification of Dengue and Zika Virus Non-Structural Protein 5 for Crystallization and Screening of Antivirals
Aicha Gharbi-Ayachi, Abbas El Sahili, and Julien Lescar
6. Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly
Szu-Chia Hsieh, Wen-Yang Tsai, and Wei-Kung Wang
7. Construction of Genomic and Sub-Genomic Dengue Infectious Replicons
Thu M. Cao and Richard J. Kuhn
Part II: Interaction between Dengue Virus and Cellular Proteins
8. Dengue Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair Correlation Analysis
Manuela V. Gabriel, Ignacio Sallaberry, Guadalupe S. Costa Navarro, Enrico Gratton, Andrea V. Gamarnik, and Laura C. Estrada
9. Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions
Jonas Nascimento Conde
10. Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry
Vivian Huerta and Yassel Ramos
Part III: Immunopathogensis and Diagnosis
11. Molecular Diagnosis Of Dengue
Priscila C.G. Nunes, Monique R.Q. Lima, and Flávia B. dos Santos
12. Serological Diagnosis of Dengue
Monique R.Q. Lima, Priscila C.G. Nunes, and Flávia B. dos Santos
13. Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies
Jonas Nascimento Conde, Megan Mladinich, William Schutt, and Erich R. Mackow
14. Evaluation of DENV-Induced Endothelial Cell Permeability by Measurements of Transendothelial Electrical Resistance (TEER) and Extravasation of Proteins and Virus
Lana Monteiro Meuren, Sharton Vinicius Antunes Coelho, and Luciana Barros de Arruda
15. In Vitro Cytokine Production by Dengue-Infected Human Monocyte-Derived Dendritic Cells
Allan Henrique Depieri Cataneo, Juliano Bordignon, Pryscilla Fanini Wowk, and Guilherme Ferreira Silveira
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsThis detailed volume explores various aspects of the dengue virus and its four serotyp. N° de réf. du vendeur 483465483
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by covering protocols to study the interactions between DENV and cellular proteins, DENV immunopathogenesis and diagnosis, as well as recent advances in animal models that can be used in studies of DENV immunopathogenesis and vaccine development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Dengue Virus: Methods and Protocols serves as an ideal guide for researchers attempting to further understand this critical threat to worldwide public health. 308 pp. Englisch. N° de réf. du vendeur 9781071618783
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Buch. Etat : Neu. Dengue Virus | Methods and Protocols | Ronaldo Mohana-Borges | Buch | xi | Englisch | 2021 | Springer US | EAN 9781071618783 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. N° de réf. du vendeur 120279006
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Buch. Etat : Neu. Neuware -This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by covering protocols to study the interactions between DENV and cellular proteins, DENV immunopathogenesis and diagnosis, as well as recent advances in animal models that can be used in studies of DENV immunopathogenesis and vaccine development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 308 pp. Englisch. N° de réf. du vendeur 9781071618783
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ISBN 13 : 9781071618783
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by covering protocols to study the interactions between DENV and cellular proteins, DENV immunopathogenesis and diagnosis, as well as recent advances in animal models that can be used in studies of DENV immunopathogenesis and vaccine development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Dengue Virus: Methods and Protocols serves as an ideal guide for researchers attempting to further understand this critical threat to worldwide public health. N° de réf. du vendeur 9781071618783
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