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Plasmodesmata: Methods and Protocols ISBN 13 : 9781071621318

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9781071621318: Plasmodesmata: Methods and Protocols

Synopsis

Part I: Overview Articles

1. Plasmodesmata Structural Components and Their Role in Signaling and Plant Development

Philip Kirk and Yoselin Benitez-Alfonso

2. Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses

Caiping Huang and Manfred Heinlein

Part II: Structure and Distribution of Plasmodesmata

3. Plasmodesmata Ultrastructure Determination Using Electron Tomography

Jules D. Petit, Marie Glavier, Lysiane Brocard, and Emmanuelle Bayer

4. Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata

Kamila Godel-Jędrychowska, Tilman Franke, and Ewa Kurczyńska

5. Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots

Andrea Paterlini and Ilya Belevich

6. Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities

Brandon C. Reagan, John R. Dunlap, and Tessa M. Burch-Smith

7. Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy

Florence R. Danila

8. Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy

Kirsten Knox

Part III: Compositional Analysis of Plasmodesmata

9. In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata

Caiping Huang, Jerôme Mutterer, and Manfred Heinlein

10. Immunofluorescence Detection of Callose in Plant Tissue Sections

Sam Amsbury and Yoselin Benitez-Alfonso

11. Callose Detection and Quantification at Plasmodesmata in Bryophytes

Arthur Muller, Tomomichi Fujita, and Yoan Coudert

12. Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis

Laetitia Fouillen, Stéphane Claverol, Emmanuelle M.F. Bayer, and Magali S. Grison

13. Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons

Verena Kriechbaumer and Stanley W. Botchway

14. Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer

Patrick Blümke, Vicky Howe, and Rüdiger Simon

15. Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy

Joseph F. McKenna

16. Using Steady-State Fluorescence Anisotropy to Study Protein Clustering

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9781071621349: Plasmodesmata: Methods and Protocols

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ISBN 10 :  1071621343 ISBN 13 :  9781071621349
Editeur : Humana, 2023
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Gebunden. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. This fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural compo. N° de réf. du vendeur 517240583

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Manfred Heinlein
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Plasmodesmata: Methods and Protocols, Second Edition serves as a vital guide for all plant scientists, both novice and expert, especially those studying the intricacies of cell-to-cell communication pathways. 484 pp. Englisch. N° de réf. du vendeur 9781071621318

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Buch. Etat : Neu. Neuware -This fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 484 pp. Englisch. N° de réf. du vendeur 9781071621318

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Manfred Heinlein
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Buch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - This fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Plasmodesmata: Methods and Protocols, Second Edition serves as a vital guide for all plant scientists, both novice and expert, especially those studying the intricacies of cell-to-cell communication pathways. N° de réf. du vendeur 9781071621318

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