Directed Evolution: Methods and Protocols - Couverture souple

Couverture souple

ISBN 10 : 1071621548 ISBN 13 : 9781071621547

Editeur : Humana, 2023

Synopsis

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

Détails de l'édition

• Éditeur: Humana
• Date d'édition: 2023
• Langue: anglais
• ISBN 10: 1071621548
• ISBN 13: 9781071621547
• Reliure: Broché
• Nombre de pages: 292
• Éditeur: Currin Andrew, Swainston Neil
• Coordonnées du fabricant: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Allemagne